Abstract:

BACKGROUND: Extracorporeal shock wave has been shown to influence the physiological function of endothelial cells via the activation of mechanoreceptors and specific signal transduction system, and gene expression regulation.
OBJECTIVE: To explore the impact of different energy flow densities and numbers of shots of extracorporeal shock waves on the new vessel formation ability, migration capability and apoptosis of bone microvascular endothelial cells.
METHODS: Bone microvascular endothelial cells isolated from the femoral head of patients undergoing arthroplasty were subcultured in vitro, and then were immunofluorescently evaluated with endothelial cell marker antibodies to CD31 and von Willebrand factor (vWF), and grouped according to different energy flow densities (low, 0.03 mJ/mm2; high, 0.11 mJ/mm2) and numbers of shots (400 and 800). Capillary-like tube formation, migration capability and apoptosis of bone microvascular endothelial cells were determined by 3-D culture in vitro, scratch test, and flow cytometry, respectively.
RESULTS AND CONCLUSION: vWF and CD31 were positively expressed in approximately 100% of bone microvascular endothelial cells, which indicates the cultured cells had characterization of microvascular endothelial cells. Extracorporeal shock wave enhanced angiogenesis and migration capability of bone microvascular endothelial cells derived from the femoral head, and especially low-energy flow density of extracorporeal shock wave exerted more superior effects. Angiogenesis of bone microvascular endothelial cells was decreased with the increased shot number in the low-energy flow density group. In addition, extracorporeal shock wave inhibited bone microvascular endothelial cell apoptosis induced by steroids. Our results suggest that energy flow density and number of shots of extracorporeal shock waves impact the physiological function of bone microvascular endothelial cells derived from the femoral head.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Physical Therapy Modalities, Microvessels, Endothelial Cells, Tissue Engineering