Effects of human umbilical cord mesenchymal stem cells via intramuscular injection on the myocardial micrangium and collagen expression in normal rats

doi:10.3969/j.issn.2095-4344.2016.28.003

Abstract

Abstract:

BACKGROUND: To date, it is still unclear whether the intramuscular injection of heterogeneous umbilical cord mesenchymal stem cells (UC-MSC) can cause cardiac ectopic pathological angiogenesis as well as increase collagen synthesis to promote myocardial fibrosis.
OBJECTIVE: To explore the effects of intramuscular injection of human UC-MSCs on myocardial micrangium and collagen expression in normal Wistar rats.
METHODS: After 2 weeks of feeding, 60 male SPF Wistar rats were randomly assigned to receive intramuscular injection of PBS (normal group), DMEM (culture medium group), human UC-MSCs supernatant (supernatant group), 0.25×10⁵, 1.0×10⁵, 4.0×10⁵ human UC-MSCs (low-, moderate- and high-dose groups), respectively (n=10 per group). All the rats were subjected to second injection (same dose) at 4 weeks after first intramuscular injection. Then, the rats were killed under anesthesia at 4 weeks after second injection, to take heart tissues from the left ventricle for pathological observation, immunohistochemical examination and Masson staining.
RESULTS AND CONCLUSION: No alteration of the response, activity, victualage, faeces, weight growth, and fur was found, and there was no death in rats during the experiment. All the rats had no symptoms of molt, inflammation, skin ulcer, scleroma. Strong positive expression of CD34 for the micrangium in the myocardial tissue was observed, and positive expression of the collagen in the myocardial tissue observed by Masson staining. There were no significant differences in the microvessel density and collagen expression in the myocardium among the groups (F=0.110 and 0.585, P > 0.05). To conclude, hUC-MSCs or its supernatant via intramuscular injection has no effect on the micrangium and collagen expression in normal rats.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Umbilical Cord, Mesenchymal Stem Cell Transplantation, Injections, Intramuscular, Microvessels, Tissue Engineering

CLC Number:

R394.2

Guo Yu-xiu, Wang Si-ping, Zhang Wen-xiang, Mao Cheng-gang, Gao Hong, Li Zi-pu. Effects of human umbilical cord mesenchymal stem cells via intramuscular injection on the myocardial micrangium and collagen expression in normal rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(28): 4123-4129.

Figures/Tables 1

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