Effect of long needle penetration needling on matrix metalloproteinase inhibitor 1 in the synovium of knee osteoarthritis model rats

doi:10.3969/j.issn.2095-4344.2016.18.004

Abstract

Abstract:

BACKGROUND: Matrix metalloproteinase inhibitor 1 plays an important role in the inhibition of articular cartilage degeneration and destruction.

OBJECTIVE: To observe the changes of matrix metalloproteinase inhibitor 1 expression level in synovium of knee osteoarthritis rats after treated with penetration needling of long needle.

METHODS: A total of 40 Sprague-Dawley rats were randomly assigned into normal group, penetration needling group, drug group and model group, with 10 rats in every group. 1.6% papain solution was injected into knee joint cavity of Sprague-Dawley rats. 2 weeks later, knee osteoarthritis models were established. Rats in the normal and model groups did not receive any intervention. In the penetration needling group, rats were treated by penetration needling with 0.3 mm×125.0 mm filiform needle. Rats in the drug group were intraarticularly injected with sodium hyaluronate.

RESULTS AND CONCLUSION: (1) Enzyme linked immunosorbent assay: at 4 weeks after treatment, matrix metalloproteinase inhibitor 1 expression in synovial membrane was higher in the normal group than in the model group (P < 0.05). Matrix metalloproteinase inhibitor 1 expression in synovial membrane was higher in the penetration needling group and drug group than in the model group (P < 0.05). (2) Results verified that long needle penetration therapy can effectively increase matrix metalloproteinase inhibitor 1 expression in synovial membrane of knee osteoarthritis model rats.
中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Models, Animal, Knee Joint

Li Shi-sheng, Wu Yao-chi, Zhang Jun-feng. Effect of long needle penetration needling on matrix metalloproteinase inhibitor 1 in the synovium of knee osteoarthritis model rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(18): 2609-2614.

Figures/Tables 2

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