Effects of bone marrow mesenchymal stem cells on the biological behavior of prostate cancer cells

doi:10.3969/j.issn.2095-4344.2016.14.010

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells have a close relationship with tumor occurrence and development, which are not only involved in tumor recruitment but impact the biological behavior of tumor cells.

OBJECTIVE: To investigate the effect of bone marrow mesenchymal stem cells on the biological behavior prostate cancer cell line.

METHODS: Human bone marrow mesenchymal stem cells from healthy adults were collected and cultured in vitro. Cell morphology and cell markers were observed. Using the Transwell technique, human dermal fibroblasts HDF-a were co-cultured with human prostate carcinoma DU145 cells in positive control group, wild-type prostate carcinoma DU145 cells cultured alone in negative control group, and bone marrow mesenchymal stem cells co-cultured with human prostate carcinoma DU145 cells in experimental group. Flow cytometry assay was used to detect the cell cycle of prostate carcinoma DU145 cells, soft agar colony formation assay performed to detect cell proliferative ability, and crystal violet staining used to observe DU145 cell migration at 3 days after culture.

RESULTS AND CONCLUSION: Compared with the negative control group, cell proportion at G₂/M+S phase, number of transmembrane cells and colony counting were significantly decreased in the experimental group. These findings indicate that bone marrow mesenchymal stem cells may inhibit the proliferation and invasion of prostate carcinoma DU145 cells by shortening the G₂/M+S phase, thereby providing a new insight into the treatment of human prostate cancer.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Prostatic Neoplasms, Cell Cycle, Cell Proliferation, Tissue Engineering

Liu Jian-jun, Duan Xiao-yu. Effects of bone marrow mesenchymal stem cells on the biological behavior of prostate cancer cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(14): 2046-2051.

Figures/Tables 1

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