Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (45): 7279-7285.doi: 10.3969/j.issn.2095-4344.2015.45.012

Previous Articles     Next Articles

Fibroblast growth factor-modified bone marrow mesenchymal stem cells promote functional recovery from traumatic brain injury

Li Xue-dong, Chen Jia-kang, Qin Jun, Mai Yong-jun, Xiao Zhen-yong   

  1. Liuzhou Workers Hospital, Liuzhou 545000, Guangxi Zhuang Autonomous Region, China
  • Received:2015-09-19 Online:2015-11-05 Published:2015-11-05
  • Contact: Chen Jia-kang, Chief physician, Liuzhou Workers Hospital, Liuzhou 545000, Guangxi Zhuang Autonomous Region, China
  • About author:Li Xue-dong, Associate chief physician, Liuzhou Workers Hospital, Liuzhou 545000, Guangxi Zhuang Autonomous Region, China

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) can promote nerve regeneration, but there are no better results because of the limitations of treatment methods. BMSC transplantation alone is not enough to achieve desired therapeutic effects.
OBJECTIVE: To investigate the effect of fibroblast growth factor (FGF)-modified BMSC transplantation on functional recovery and expression of glial fibrillary acidic protein after traumatic brain injury.
METHODS: Animal models of traumatic brain injury were established in Sprague-Dawley rats using hydraulic shock method, and then randomized into control group (traumatic brain injury group), BMSC group and FGF-BMSC group (FGF-modified BMSC group). After isolation and culture, BMSCs were modified by adenovirus vector-mediated FGF gene. Western blot assay was used to detect transfection efficiency and glial fibrillary acidic  
protein expression; immunohistochemical detection was used to detect distribution and number of BrdU positive cells in the brain; Longa score was used to evaluate the neurologic function of rats at 1, 3 days, 1, 2 weeks after transplantation; TUNEL assay was used to detect cell apoptosis in the brain.
RESULTS AND CONCLUSION: Western blot results showed that FGF gene was successfully transferred to the adenovirus vector, and capable of expressing in BMSCs; moreover, the glial fibrillary acidic protein expression of FGF-BMSC group was significantly higher than that in the other two groups (P < 0.05). The number of BrdU positive cells in the brain was significantly higher in the FGF-BMSC group than the other two groups (P < 0.05). Two weeks after transplantation, the Longa scores in the FGF-BMSC group were significantly lower than those in the other two groups (P < 0.05). TUNEL results showed that the number of apoptotic cells in the FGF-BMSC group was significantly lower than that in the other two groups (P < 0.05). These findings indicate that FGF-modified BMSCs transplantation is able to improve neurological damage after traumatic brain injury and promote neurological recovery, which is better than BMSC transplantation alone. 
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Mesenchymal Stem Cells, Fibroblast Growth Factors, Transfection, Tissue Engineering