Effect of human umbilical cord mesenchymal stem cells on the proliferative characteristics of cervical cancer Hela cells

doi:10.3969/j.issn.2095-4344.2015.45.011

Abstract

Abstract:

BACKGROUND: Mesenchymal stem cells have the specific chemotaxis to the inflammation and tumor tissue, but the effect of mesenchymal stem cells on the growth of cervical cancer cells becomes an urgent problem.
OBJECTIVE: To investigate the effect of human umbilical cord mesenchymal stem cells on the proliferation of cervical cancer Hela cells.
METHODS: Hela cells were co-cultured with human umbilical cord mesenchymal stem cells at different number or its conditioned medium at different concentrations for 3 days. Then, cell counting kit-8 was used to detect the proliferation of Hela cells.
RESULTS AND CONCLUSION: When Hela cells were co-cultured with human umbilical cord mesenchymal stem cells at ratios of 1:3, 1:2, 1:1, 2:1, 4:1, 8:1, the relative proliferation inhibition rates were 67.12%, 47.18%, 31.15%, 27.61%, 15.55% and 15.95%, respectively. When the Hela cells were co-cultured with human umbilical cord mesenchymal stem cell conditioned medium at 5, 10, 20, 40, 60, 100 mg/L, the relative proliferation inhibition rates were 0.61%, 40.1%, 63.47%, 80.61%, 93.56%, 90.65%, respectively. These findings indicate that the proliferation of Hela cells can be inhibited by co-culture with human umbilical cord mesenchymal stem cells at a certain concentration-dependent manner.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Umbilical Cord, Mesenchymal Stem Cells, Uterine Cervical Neoplasms, Hela Cells, Tissue Engineering

Figures/Tables 5

References

[1] 刘华,陈亚娜,郑燕,等.人脐带间充质干细胞对宫颈癌Hela细胞增殖的影响[J].山东大学学报:医学版,2013,51(6):29-33,39.

[2] 林琳,孙雯,王丽双,等.间充质干细胞对人HeLa宫颈癌细胞生长的影响[J].中华医学杂志,2015,95(15):1175-1178.

[3] 张松灵,张海鹏,王艳丽,等.正常宫颈及宫颈癌组织中干细胞标记物表达情况的研究[J].中国妇幼保健,2008,23(3):392-393.

[4] Yang S, Xue DD, Wu B, et al. Pleiotrophin is involved in the amniotic epithelial cell-induced differentiation of human umbilical cord blood-derived mesenchymal stem cells into dopaminergic neuron-like cells. Neurosci Lett. 2013;539:86-91.

[5] 黎娇,朱争艳,杜智,等.人脐带间充质干细胞分泌物对肝细胞增殖和凋亡的影响[J].中华肝胆外科杂志,2010,16(6):460-464.

[6] 朱少芳,何援利,付霞霏,等.人脐带间充质干细胞的生物学特性和超微结构[J].中国医学科学院学报,2011,33(4):382-386.

[7] 张铭,张一鸣,左伟,等.干细胞标志物SALL4在宫颈癌中的表达研究[J].重庆医学,2014,43(3):285-287.

[8] 王雪莉,曹晓诚,盛习锋,等.紫花牡荆素对宫颈癌HeLa细胞系干细胞样细胞自我更新的影响[J].湖南师范大学学报:医学版,2013, 10(3):11-14.

[9] Bai H.,Chen P,Tang GQ, et al. Relations between reactive oxygen species and Raman spectral variations of human umbilical cord mesenchymal stem cells with different viability [J].Laser Physics. 2011;21(6):1122-1129.

[10] 唐秋英,黄平,金瑛,等.干细胞基因Oct4和Bcl-2在宫颈癌及其癌前病变组织中的表达及意义[J].海南医学,2013,24(5):625-627.

[11] 张松灵,张立红,钟艳平,等.人宫颈癌组织中CK17和P63蛋白的表达及意义[J].吉林大学学报:医学版,2009,35(5):914-917.

[12] Ferrandina G, Perillo A, Distefano M, et al. Carboplatin-based neoadjuvant treatment with peripheral blood stem cell and growth factor support in locally advanced cervical cancer patients with bulky metastatic lymph nodes. Eur J Obstet Gynecol Reprod Biol. 2007;131(2):236-238.

[13] Simões IN, Boura JS, dos Santos F, et al. Human mesenchymal stem cells from the umbilical cord matrix: successful isolation and ex vivo expansion using serum-/xeno-free culture media. Biotechnol J. 2013;8(4):448-458.

[14] 韩琪,李斌,王克芳,等.人宫颈癌干细胞的分离鉴定[J].中国妇幼保健,2013,28(4):681-684.

[15] Esposito M, Lucariello A, Costanzo C, et al. Differentiation of human umbilical cord-derived mesenchymal stem cells, WJ-MSCs, into chondrogenic cells in the presence of pulsed electromagnetic fields. In Vivo. 2013;27(4):495-500.

[16] 张松灵,张海鹏,王建六,等.宫颈癌Hela细胞株中Bcrp1+表型细胞的侵袭力[J].中国老年学杂志,2012,32(7):1417-1419.

[17] 马衣努尔﹒买提托合提.上皮间质化(EMT)在宫颈癌发生发展中诱导肿瘤干细胞的作用及其机制研究[D].武汉:华中科技大学, 2013.

[18] 曹晓正,杨小红,曹建国,等.8-溴-7-甲氧基白杨素逆转人宫颈癌SiHa细胞系球形成细胞上皮-间叶样表型转化[J].中国药理学通报,2014,30(5):668-671.

[19] Montesinos JJ, Mora-García Mde L, Mayani H, et al. In vitro evidence of the presence of mesenchymal stromal cells in cervical cancer and their role in protecting cancer cells from cytotoxic T cell activity. Stem Cells Dev. 2013;22(18):2508- 2519.

[20] 宋菁华,王克芳,李斌,等.人宫颈癌侧群细胞的分选及其生物学特性的研究[J].首都医科大学学报,2012,33(1):5-10.

[21] Rocha-Zavaleta L, Huitron C, Cacéres-Cortés JR, et al. Interleukin-2 (IL-2) receptor-betagamma signalling is activated by c-Kit in the absence of IL-2, or by exogenous IL-2 via JAK3/STAT5 in human papillomavirus-associated cervical cancer. Cell Signal. 2004;16(11):1239-1247.

[22] 陈秀慧,曲军英.不同质量浓度顺铂对宫颈癌 Hela 细胞增殖能力的影响[J].中国临床药理学杂志,2014,30(7):601-602.

[23] 陈道桢,王常在,陈万瑛,等.载20(S)-人参皂苷白蛋白纳米微球对人宫颈癌Hela细胞生长抑制作用研究[J].中国实验诊断学,2013, 17(8):1394-1397.

[24] Wang F, Zhang YC, Zhou H, et al. Evaluation of in vitro and in vivo osteogenic differentiation of nano-hydroxyapatite/ chitosan/poly(lactide-co-glycolide) scaffolds with human umbilical cord mesenchymal stem cells. J Biomed Mater Res A. 2014;102(3):760-768.

[25] Liu S, Hou KD, Yuan M, et al. Characteristics of mesenchymal stem cells derived from Wharton's jelly of human umbilical cord and for fabrication of non-scaffold tissue-engineered cartilage. J Biosci Bioeng. 2014;117(2):229-235.

[26] Roszek K, Bomastek K, Dro?d?al M, et al. Dramatic differences in activity of purines metabolizing ecto-enzymes between mesenchymal stem cells isolated from human umbilical cord blood and umbilical cord tissue. Biochem Cell Biol. 2013;91(6):519-525.

[27] 赵雯红,程建新,史鹏飞,等.转染IL-12基因的人脐带间充质干细胞对卵巢癌细胞的体外增殖和对裸鼠体内移植瘤的抑制作用[J].南方医科大学学报,2011,31(5):903-907.

[28] 马懿,郝晓甍,张晟,等.人脐带间充质干细胞对乳腺癌干细胞影响的体内外研究[C].第二届中国女医师大会论文集,2012:237- 240.

[29] Studeny M, Marini FC, Champlin RE, et al. Bone marrow-derived mesenchymal stem cells as vehicles for interferon-beta delivery into tumors. Cancer Res. 2002;62(13): 3603-3608.