Expression of sterol regulatory element-binding protein-2 in the process of chondrocyte degeneration

doi:10.3969/j.issn.2095-4344.2015.29.007

Abstract

Abstract:

BACKGROUND: Recent studies have demonstrated that sterol regulatory element binding protein-2 (SREBP-2) plays a key role in osteoarthritis, but its exact pathogenesis remains incompletely understood yet.
OBJECTIVE: To investigate the expression of SREBP-2 in the process of interleukin-1β-induced articular chondrocyte degeneration in vitro.
METHODS: Articular chondrocytes obtained from C57BL/6J mice were cultured in vitro. After the second passage, cells were randomly divided into four groups: control group, and three experimental groups treated with 10 μg/L interleukin-1β for 24, 48 and 72 hours, respectively.
RESULTS AND CONCLUSION: The cells became hypertrophic after being stimulated by interleukin-1β, and the staining of collagen X was positive at 72 hours. MTT assay demonstrated that the cell activity after stimulation with interleukin-1β decreased with time. Results of RT-PCR showed that the expression of SREBP-2 and SREBP cleavage activating protein mRNA was significantly increased after stimulation with interleukin-1β as compared with the control group and increased with time. On the contrary, the expression of aggrecan and collagen II mRNA was decreased with time. It is revealed that interleukin-1β could inhibit the proliferation of regular chondrocytes and the expression of its extracellular matrix, and furthermore, induce chondrocyte hypertrophy. The expression of SREBP-2 showed a negative relationship with key cartilage genes during this interleukin-1β-induced degeneration.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Sterol Regulatory Element Binding Proteins, Interleukins, Chondrocytes, Osteoarthritis

CLC Number:

R318

Weng Jian, Zeng Hui, Xiao De-ming, Tao Ke, Kang Bin, Liang Hao-feng. Expression of sterol regulatory element-binding protein-2 in the process of chondrocyte degeneration[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(29): 4624-4628.

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