In vivo osteogenesis by implanting dual gene activated nanobone putty

doi:10.3969/j.issn.2095-4344.2014.03.001

Abstract

Abstract:

BACKGROUND: The bone morphogenetic protein 2 (BMP2)/vascular endothelial growth factor (VEGF) dual gene activated nanobone putty has been constructed in the previous experiments.
OBJECTIVE: To investigate the effects of osteogenesis and osteogenic gene expression in mice by implanting BMP2/VEGF dual gene activated nanobone putty.
METHODS: Twenty-four Kunming mice (48 sides) were randomly divided into four groups. Animals in each group (12 samples) were injected different materials into the right thigh muscle pouches: nanobone putty+hBMP2/VEGF plasmid; nanobone putty+hBMP2 plasmid; blank plasmid+nanobone putty; nanobone putty only. The effects of osteogenesis were evaluated by radiography, histology and molecular biology analysis in 2, 4 weeks after operation.
RESULTS AND CONCLUSION: Bone-like tissues were observed in groups of nanobone putty+hBMP2/VEGF plasmid and nanobone putty+hBMP2 plasmid after operation. There was apparent BMP2 and VEGF mRNA expression in group of nanobone putty+hBMP2/VEGF plasmid. Group of nanobone putty+hBMP2/VEGF plasmid was significantly better than group of nanobone putty+hBMP2 plasmid in the alkaline phosphatase levels, the speed of osteogenesisas and amount of new bone (P < 0.05). Groups of blank plasmid+nanobone putty and nanobone putty had no obvious osteogenesis performance. Either BMP2/VEGF dual gene activated nanobone putty or BMP2 gene activated nanobone putty had the osteogenic ability in vivo. And the former was significantly enhanced in the speed and quality of osteogenesis.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

全文链接：

Key words: biocompatible materials, nanoparticles, genes, bone morphogenetic proteins

CLC Number:

R318

Zhang Yi, Sun Li, Jian Yue-kui, Hu Ru-yin, Tian Xiao-bin, Li Bo, Han Wei. In vivo osteogenesis by implanting dual gene activated nanobone putty[J]. Chinese Journal of Tissue Engineering Research, 2014, 18(3): 329-334.

Figures/Tables 5

References

[1]陈红卫,赵钢生,叶键.自固化磷酸钙人工骨修复不同病因骨缺损94例[J].中国组织工程研究与临床康复, 2008,12(19): 3779-3781.

[2]Semino CE.Self-assembling peptides:from bio-inspired materials to bone regeneration.J Dent Res.2008;87(7): 606-616.

[3]Garreta E,Gasset D,Semino C,et al.Fabrication of a three-dimensional nanostructured biomaterial for tissue engineering of bone.Biomol Eng.2007;24(1):75-80.

[4]Reves BT,Bumgardner JD,Haggard WO.Fabrication of crosslinked carboxymethylchitosan microspheres and their incorporation into composite scaffolds for enhanced bone regeneration.J Biomed Mater Res B Appl Biomater. 2013; 101(4):630-639.

[5]Li B,Yoshii T,Hafeman AE,et al.The effects of rhBMP-2 released from biodegradable polyurethane/microsphere composite scaffolds on new bone formation in rat femora. Biomaterials.2009;30:6768-6779.

[6]Zou D,Guo L,Lu J,et al.Engineering of bone using porous calcium phosphate cement and bone marrow stromal cells for maxillary sinus augmentation with simultaneous implant placement in goats.Tissue Eng Part A.2012;18:1464-1478.

[7]罗萍,张阳德,彭健,等.具有自塑能力可吸收注射型纳米骨浆[J].中国现代医学杂志, 2003,13(18):1-6.

[8]王明海,董有海,洪洋,等.纳米组织工程骨对骨缺损修复区骨密度及生物力学的影响[J].中国临床医学杂志,2007,14(5):678-680.

[9]田晓滨,孙立,杨述华.克隆并构建人骨形成发生蛋白2真核表达载体[J].中国修复重建外科杂志,2006,20(2):112-115.

[10]Parsons B,Strauss E.Surgical management of chronic osteomyelitis.Am J Surg.2004;188:57-64.

[11]Sasso RC,Williams JI,Dimasi N,et al.Postoperative drains at the donor site Of iliac-crest bone graft.J Bone and Joint Surg [Am].1998;80(5):631-635.

[12]Kohara H,Tabata Y.Enhancement of ectopic osteoid formation following the dual release of bone morphogenetic protein 2 and Wnt1 inducible signaling pathway protein 1 from gelatin sponges.Biomaterials.2011;32(24):5726.

[13]Keibl C,Fügl A,Zanoni G,et al. Human adipose derived stem cells reduce callus volume upon BMP-2 administration in bone regeneration.Injury.2011; 42(8):814.

[14]Sawyer AA,Song SJ,Susanto E,et al.The stimulation of healing within a rat calvarial defect by mPCL-TCP/collagen scaffolds loaded with rhBMP-2. Biomaterials.2009; 30: 2479-2488.

[15]Zhao J,Shen G,Liu C,et al.Enhanced healing of rat calvarial defects with sulfated chitosan-coated calcium-deficient hydroxyapatite/bone morphogenetic protein 2 scaffolds. Tissue Eng Part A.2012;18:185-197.

[16]Crouzier T,Sailhan F,Becquart P,et al.The performance of BMP-2 loaded TCP/HAP porous ceramics with a polyelectrolyte multilayer film coating. Biomaterials. 2011; 32:7543-7554.

[17]Grauer JN,Beiner JM,Kwon BK,et al.Bone graft alternatives for spinal fusion. BioDrugs.2003;17:391-394.

[18]Bonadio J.Tissue engineering via local gene delivery: update and future prospects for enhancing the technology.Adv Drug Deliv Rev.2000;44:185-194.

[19]田晓滨,孙立,杨述华,等.基因活化纳米骨浆异位诱导成骨能力的实验观察[J].中华骨科杂志,2007,27(8):604-608.

[20]Deckers MM,van Bezooijen RL,van der Horst G,et al.Bone morphogenetic proteins stimulate angiogenesis through osteoblast-derived vascular endothelial growth factor A. Endocrinology.2002;143(4):1545-1553.

[21]Bouletreau PJ,Warren SM,Spector JA,et al.Hypoxia and VEGF up-regulate BMP-2 mRNA and protein expression in microvascular endothelial cells:implications for fracture healing.Plast Reconstr Surg.2002;109(7):2384-2397.