Concentrated red bone marrow/platelet-rich fibrin compound with an autologous periosteum debris scaffold for repair of mandibular defects

doi:10.3969/j.issn.2095-4344.2013.21.006

Abstract

Abstract:

BACKGROUND: The platelet-rich fibrin scaffold structure is conducive to the growth of red bone marrow mesenchymal stem cells and various growth factors, promoting final osteogenesis.
OBJECTIVE: To investigate the feasibility of the concentrated red bone marrow/platelet-rich fibrin compound with the autologous periosteum debris scaffold for repair of rabbit mandibular defects.
METHODS: Bilateral mandibular bone defect models were prepared in New Zealand white rabbits using self-control method. The left side was the experimental side, and implanted with the concentrated red bone marrow/platelet-rich fibrin compound with the autologous periosteum debris scaffolds; the right side was the control side, and implanted with periosteum fragments and nano-hydroxyapatite composite scaffolds. All experimental animals were sacrificed after 2, 4, 8, 12 weeks to prepare tissue samples for general observation, imaging analysis, hematoxylin-eosin staining and scanning electron microscope detection.
RESULTS AND CONCLUSION: The imaging examination and histological staining showed that the experimental side was superior to the control side in the aspects of bone healing, osteogenesis speed and quality. Under the scanning electron microscope, the experimental side had better compatibility with surrounding tissues and no inflammation reaction. The data of the tooth CT and evidence of new bone formation showed the bone density and new bone area of the experimental side were significantly higher than that of the control side (P < 0.05). These data indicate that concentrated red bone marrow/platelet-rich fibrin compound with the autologous periosteum debris scaffolds has an obvious osteoinductive role, which is expected to be a new-type material for clinical repair of mandibular defects.

Key words: biomaterials, tissue-engineered bone materials, concentrated red bone marrow, platelet-rich fibrin, nano-hydroxyapatite, bone, mandibular defects, autologous periosteum

CLC Number:

R318

Guo Ling-yu, Liu Kai, Liu Shu-fa. Concentrated red bone marrow/platelet-rich fibrin compound with an autologous periosteum debris scaffold for repair of mandibular defects[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.21.006.

Figures/Tables 4

2.1 实验动物数量分析 36只兔均进入结果分析。 2.2 实验组与对照组术后不同时间点骨缺损处大体观察结果术后2周时，实验组与对照组骨缺损区均有纤维状组织包裹，对照组多见炎性渗出，两组材料均有小部分吸收，与周围骨界限清，探针检测质地较软。术后4周时，实验组材料吸收明显，缺损边缘与周边骨结合较牢固，探测质地较硬；对照组纤维组织充填较多，质地软。术后8周时，实验组材料大部吸收，缺损区与周围骨无明显差异，硬度明显增加；对照组残存材料较多，硬度不及实验组。术后12周时，实验组缺损区骨膜包被，硬度与周围骨相当；对照组表面仍有纤维组织，硬度不明显。 2.3 实验组与对照组术后不同时间点骨缺损处X射线检测结果术后2周时，实验组与对照组低密度影明显，有材料阻射影，与周围骨组织界清。术后4周时，实验组可见絮状高密度影，材料可见吸收，硬度增加，与周围正常骨边界尚清；对照组缺损区材料阻射影依旧清楚，低密度影多，与周围界限清。术后8周时，实验组区域可见均匀骨痂影像，有骨性连接，骨密度增加，与周围界限不清；对照组缺损区可见絮状高密度影，骨密度较低，与周围界限尚清。术后12周时，实验组缺损区与周围骨无差异，界限模糊不清，密度与正常骨大致相等；对照组仍见低密度影，密度依旧较低，与周围界限尚清，见图3。 2.4 实验组与对照组术后不同时间点骨缺损处扫描电镜检测结果术后2周时，两组缺损区材料均与周围骨界限有裂隙。术后4周时，实验组材料与周围骨结合较紧，界限不清，有纤维组织长入，骨痂形成；对照组界限清楚，少量胶原纤维长入。术后8周时实验组材料与周围骨界限模糊，大量骨痂形成；对照组缺损区材料与周围骨界限不明显，少量骨痂形成。术后12周时，实验组材料与周围骨缺损区界限不清，形成大片生长良好的新生骨；对照组界限尚清，仍见残存材料，周缘有钙化，见图4。"

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