Chinese Journal of Tissue Engineering Research
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Guo Ling-yu, Liu Kai, Liu Shu-fa
Received:
2012-09-14
Revised:
2012-10-09
Online:
2013-05-21
Published:
2013-05-21
Contact:
Liu Kai, Master, Attending physician, Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jiamusi University, Jiamusi 154000, Heilongjiang Province, China
lkdx1980@163.com
About author:
Guo Ling-yu★, Studying for master’s degree, Department of Oral and Maxillofacial Surgery, Hospital of Stomatology, Jiamusi University, Jiamusi 154000, Heilongjiang Province, China
yuling861012@sina.com
CLC Number:
Guo Ling-yu, Liu Kai, Liu Shu-fa. Concentrated red bone marrow/platelet-rich fibrin compound with an autologous periosteum debris scaffold for repair of mandibular defects[J]. Chinese Journal of Tissue Engineering Research, doi: 10.3969/j.issn.2095-4344.2013.21.006.
2.1 实验动物数量分析 36只兔均进入结果分析。 2.2 实验组与对照组术后不同时间点骨缺损处大体观察结果 术后2周时,实验组与对照组骨缺损区均有纤维状组织包裹,对照组多见炎性渗出,两组材料均有小部分吸收,与周围骨界限清,探针检测质地较软。术后4周时,实验组材料吸收明显,缺损边缘与周边骨结合较牢固,探测质地较硬;对照组纤维组织充填较多,质地软。术后8周时,实验组材料大部吸收,缺损区与周围骨无明显差异,硬度明显增加;对照组残存材料较多,硬度不及实验组。术后12周时,实验组缺损区骨膜包被,硬度与周围骨相当;对照组表面仍有纤维组织,硬度不明显。 2.3 实验组与对照组术后不同时间点骨缺损处X射线检测结果 术后2周时,实验组与对照组低密度影明显,有材料阻射影,与周围骨组织界清。术后4周时,实验组可见絮状高密度影,材料可见吸收,硬度增加,与周围正常骨边界尚清;对照组缺损区材料阻射影依旧清楚,低密度影多,与周围界限清。术后8周时,实验组区域可见均匀骨痂影像,有骨性连接,骨密度增加,与周围界限不清;对照组缺损区可见絮状高密度影,骨密度较低,与周围界限尚清。术后12周时,实验组缺损区与周围骨无差异,界限模糊不清,密度与正常骨大致相等;对照组仍见低密度影,密度依旧较低,与周围界限尚清,见图3。 2.4 实验组与对照组术后不同时间点骨缺损处扫描电镜检测结果 术后2周时,两组缺损区材料均与周围骨界限有裂隙。术后4周时,实验组材料与周围骨结合较紧,界限不清,有纤维组织长入,骨痂形成;对照组界限清楚,少量胶原纤维长入。术后8周时实验组材料与周围骨界限模糊,大量骨痂形成;对照组缺损区材料与周围骨界限不明显,少量骨痂形成。术后12周时,实验组材料与周围骨缺损区界限不清,形成大片生长良好的新生骨;对照组界限尚清,仍见残存材料,周缘有钙化,见图4。"
2.5 实验组与对照组术后不同时间点骨缺损处组织学检测结果 术后2周时,实验组缺损区内可见大量成骨细胞长入,细胞呈多角形,少量成纤维细胞和炎细胞浸润,红染骨基质明显;对照组可见大量成纤维细胞,炎细胞,少量成骨细胞,材料未见溶解吸收。术后4周时,实验组可见材料部分吸收,骨小梁致密成熟,可见丰富骨髓组织及骨陷窝;对照组材料吸收不明显,成骨细胞生成数量少,材料与周围界限清。术后8周时,实验组可见大量新生骨,骨小梁生成,材料吸收明显;对照组缺损区多见纤维组织和散在新生骨。术后12周时,实验组材料基本吸收,骨小梁形成规则,密度增大,皮质骨样结构增多,可见成熟骨外板和哈佛氏系统,生成骨髓组织内可见多样细胞;对照组材料残存,边界不清,材料内部多见纤维组织板状骨少见,多见纤维骨痂,骨小梁形成不规则,新生骨面积小,见图5。"
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