Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (45): 8486-8490.doi: 10.3969/j.issn.2095-4344.2012.45.023

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Xianlinggubao affects bone mass and osteogenic differentiation of bone marrow stromal cells in rats

Xing Lei1, Jiao Ying-hua2, Tian Fa-ming3   

  1. 1Department of Gerontology, 2Department of Integrated Traditional Chinese and Western Medicine, Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China; 3Medical Research Center, Hebei United University, Tangshan 063000, Hebei Province, China
  • Received:2012-01-31 Revised:2012-04-01 Online:2012-11-04 Published:2012-11-04
  • Contact: Tian Fa-ming, M.D., Lecturer, Medical Research Center, Hebei United University, Tangshan 063000, Hebei Province, China tfm9911316@163.com
  • About author:Xing Lei★, Master, Attending physician, Department of Gerontology, Affiliated Hospital of Hebei United University, Tangshan 063000, Hebei Province, China xlpangdun001@163.com

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Abstract:

BACKGROUND: Bone loss in rats can be achieved by bilateral ovariectomy. Xianlinggubao as a Chinese agent has been shown to promote bone formation.
OBJECTIVE: To investigate the effects of Xianlinggubao on bone mass and the expression level of genes related to osteogenic differentiation of bone marrow stromal cells in rats.
METHODS: Twenty-four 3-month-old Sprague-Dawley rats were randomly divided into three groups, with eight rats in each group. Rats from the ovariectomy group and Xianlinggubao group underwent ovariectomy. At 6 weeks after ovariectomy, Xianlinggubao group rats were daily administered 250 mg/kg Xianlinggubao for a total of 8 weeks. Rats from the normal control group received no intervention.
RESULTS AND CONCLUSION: The bone mineral density at L2-4 vertebral body in the Xianlinggubao group was significantly higher than that in the ovariectomy group, but it was significantly lower than that in the normal control group (both P < 0.05). Serum osteocalcin, bone morphogenetic protein 2, osteocalcin mRNA expression levels in the ovariectomy group were significantly lower than those in the Xianlinggubao group and normal control group (both P < 0.05). Extracellular matrix mineralization ability in the ovariectomy group was obviously lower than that in the Xianlinggubao group and normal control group. At 14 weeks after ovariectomy, rat bone loss was obvious, and Xianlinggubao can partly prevent bone loss. The underlying mechanism is likely to be related to Xianlinggubao promotion of osteogenic differentiation of rat bone marrow stromal cells.

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