Abstract:

BACKGROUND: Basic fibroblast growth factors have been widely used for clinical autologous fat grafting because they exhibit great potential to promote angiogenesis and adipocyte proliferation and differentiation.
OBJECTIVE: To investigate the effects of different concentrations of basic fibroblast growth factors on proliferation of adipose-derived stem cells and analyze the proper concentration of basic fibroblast growth factors for promoting proliferation of adipose-derived stem cells.
METHODS: Adipose-derived stem cells were isolated from adipose tissue by liposuction. Cell morphology and growth characteristics were observed under the microscope. Culture medium containing 0, 5, 10, 20, 40, 80, 60 μg/L basic fibroblast growth factors was prepared. Adipose-derived stem cells were cultured in a 96-well plate at 103 cells/well using culture medium containing basic fibroblast growth factors. The proliferation of adipose-derived stem cells was determined by MTT for successive 7 days.
RESULTS AND CONCLUSION: Basic fibroblast growth factors could promote the proliferation of adipose-derived stem cells significantly. The proliferation-promoting effect was significant since the 3rd day and peaked on the 5th and 6th days. There was no obvious difference in proliferation-promoting effects between different basic fibroblast growth factor concentrations. 5 μg/L was the most appropriate concentration for basic fibroblast growth factors in promoting the proliferation of adipose-derived stem cells.