Chinese Journal of Tissue Engineering Research ›› 2026, Vol. 30 ›› Issue (31): 8092-8099.doi: 10.12307/2026.450

Previous Articles     Next Articles

Preparation and performance evaluation of essence with mesenchymal stem cell conditioned medium

Wang Quansheng1, 2, 3, Chen Songtao1, 2, 3, Zhang Yongqing1, 2, 3, Qiao Hua1, 2, 3   

  1. 1Department of Biochemistry and Molecular Biology, School of Basic Medicine, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China; 2Ministry of Education Key Laboratory of Coal Environmental Pathogenicity and Prevention, Taiyuan 030001, Shanxi Province, China; 3Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Taiyuan 030001, Shanxi Province, China
  • Received:2025-12-01 Accepted:2026-02-02 Online:2026-11-08 Published:2026-05-22
  • Contact: Qiao Hua, MD, Associate professor, Master’s supervisor, Department of Biochemistry and Molecular Biology, School of Basic Medicine, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China; Ministry of Education Key Laboratory of Coal Environmental Pathogenicity and Prevention, Taiyuan 030001, Shanxi Province, China; Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Taiyuan 030001, Shanxi Province, China
  • About author:Wang Quansheng, MS candidate, Department of Biochemistry and Molecular Biology, School of Basic Medicine, Shanxi Medical University, Taiyuan 030001, Shanxi Province, China; Ministry of Education Key Laboratory of Coal Environmental Pathogenicity and Prevention, Taiyuan 030001, Shanxi Province, China; Shanxi Key Laboratory of Birth Defect and Cell Regeneration, Taiyuan 030001, Shanxi Province, China
  • Supported by:
    Shanxi Provincial Undergraduate Innovation and Entrepreneurship Program, No. 20240411 (to QH); Shanxi Provincial Natural Science Foundation Project, No. 202203021211232 (to QH)

PDF

10

Abstract: BACKGROUND: Mesenchymal stem cell conditioned medium contains a variety of bioactive substances, and has the potential to slow down the aging of skin fibroblasts. However, there are few studies on the practical application of mesenchymal stem cell conditioned medium.
OBJECTIVE: To prepare and evaluate the performance of an essence containing human umbilical cord mesenchymal stem cell-conditioned medium. 
METHODS: The formulas of mesenchymal stem cell conditioned medium essence were screened by orthogonal experiment design using sensory performance score. The stability of the essence was evaluated through storage stability test, thermal cycling stability test, and centrifugal stability test at 4°C. The safety of the essence was evaluated from three levels: hemolysis test, cytotoxicity test, and skin irritation test. The moisturizing and anti-aging effects of the serum were investigated by measuring water loss rate using moisturizing test, cell viability using the CCK-8 assay, and reactive oxygen species levels using human skin fibroblasts and a zebrafish embryo aging model. 
RESULTS AND CONCLUSION: (1) Orthogonal experimental design showed that when the addition of mesenchymal stem cell conditioned medium was 5%, glycerol was 5%, and xanthan gum was 0.25%, the sensory performance score was the highest, and the formula had good repeatability and high feasibility. (2) The mesenchymal stem cell conditioned medium essence had good stability under the conditions of 4 °C storage, hot and cold cycling and centrifugation condition, and low water loss rate. (3) The mesenchymal stem cell conditioned medium essence had good hemocompatibility, low cytotoxicity, and no skin irritation, and was safe and reliable. (4) The mesenchymal stem cell conditioned medium essence could promote the proliferation of senescent skin fibroblasts cells and reduce the levels of reactive oxygen species in aging model cells and zebrafish embryos, indicating certain anti-aging, antioxidant effects, and moisturizing properties. The above results show that the essence prepared with mesenchymal stem cell conditioned medium as the functional ingredient has good stability, is mild and non-irritating, and has anti-aging, moisturizing, and antioxidant effects.

Key words: mesenchymal stem cells, conditioned medium, replicative senescence, anti-aging, moisturizing, antioxidant, essence, human umbilical cord mesenchymal stem cells, stem cell derivatives

CLC Number: