Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (32): 6037-6041.doi: 10.3969/j.issn.2095-4344.2012.32.028

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Transplantation of induced pluripotent stem cells for cardiac rhythm in mice with acute myocardial infarction

Wei Xin-wei1, Zhang Xiao-gang2, Yang Liang3   

  1. 1First Department of Cardiology, People’s Hospital of Anyang, Anyang 455000, Henan Province, China;
    2Department of Cardiology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China;
    3ICU of Department of Internal Medicine, Third People’s Hospital of Chengdu, Chengdu 610000, Sichuan Province, China
  • Received:2012-02-21 Revised:2012-02-21 Online:2012-08-05 Published:2012-08-05
  • Contact: Zhang Xiao-gang, M.D., Professor, Chief physician, Master’s supervisor, Department of Cardiology, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China
  • About author:Wei Xin-wei★, Master, Attending physician, First Department of Cardiology, People’s Hospital of Anyang, Anyang 455000, Henan Province, China wxwzs720504@163.com

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Abstract:

BACKGROUND: Induced pluripotent stem cells have been considered as a most promosing method for treatment of ischemic heart disease. However, the safety and efficiency of transplantation of induced pluripotent stem cells require further investigation.
OBJECTIVE: To investigate the effects of induced pluripotent stem cells transplantation on cardiac rhythm of mice with acute myocardial infarion.
METHODS: Acute myocardial infarction mouse models were established and were then randomly divided into acute myocardial infarction group, acute myocardial infarction + saline group, acute myocardial infarction + induced pluripotent stem cells group, and acute myocardial infarction + fibroblasts group. At the same time, a normal control group was designated. The change in the cardiac rhythm of the limb lead II surface electrocardiogram of every group was examined by BL-420 biological function system at 5 minutes, 1, 2, 3 weeks after transplantation of induced pluripotent stem cells. The expression of connexin 43 in each group was detected by immunohistochemical staining and semi-quantitatively analyzed using Image Proplus software.
RESULTS AND CONCLUSION: Compared with acute myocardial infarction and acute myocardial infarction + fibroblast groups, the ratio of ventricular premature in the acute myocardial infarction + induced pluripotent stem cells group was obviously shortened and connexin 43 expression was significantly increased (P < 0.05). There was no significant difference between acute myocardial infarction group and acute myocardial infarction + fibroblast group. At 2 weeks after transplantation of induced pluripotent stem cells, the ratio of ventricular premature was significantly decreased, thereby the electrical activity of myocardial tissue was improved and the potential stability was enhanced, which leads to increase in connexin 43 expression. However, this phenomenon was not observed in mice receiving transplantation of fibroblasts.

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