Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (32): 6042-6046.doi: 10.3969/j.issn.2095-4344.2012.32.029

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Mechanism of CD28-B7 co-stimulatory pathway to myasthenia gravis treated by peripheral blood mononuclear cells

Liu Hong-yan, Guo Jing-ming, Wang Hai-yan, Ye Song, Ran Chang-li   

  1. Department of Hematology, Yichang Central People's Hospital, the First College of Clinical Medical Science, China Three Gorges University, Three Gorges University Oncology Institute, Yichang 443003, Hubei Province, China
  • Received:2012-02-03 Revised:2012-07-08 Online:2012-08-05 Published:2012-08-05
  • About author:Liu Hong-yan★, Master, Associate chief physician, Department of Hematology, Yichang Central People's Hospital, The First College of Clinical Medical Science, China Three Gorges University, Three Gorges University Oncology Institute, Yichang 443003, Hubei Province, China liuhy518@163.com

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Abstract:

BACKGROUND: There is evidence that CD28-B7 co-stimulatory pathway is closely related to myasthenia gravis. It remains unclear regarding the mechanism of CD28-B7 co-stimulatory pathway to myasthenia gravis treated by peripheral blood mononuclear cells.
OBJECTIVE: To investigate the expression of CD28-B7 co-stimulatory pathway in a rat model of myasthenia gravis.
METHODS: Forty female Lewis rats were divided into control group, model group, adjuvant group and stem cell transplantation group. Rats in the latter three groups were intraperitoneally administered serum from patients with myasthenia gravis to prepare myasthenia gravis models. Rats in the stem cell transplantation group were subcutaneously administered granulocyte colony stimulating factor 10 μg/kg daily for 5 successive days to mobilize bone marrow hematopoietic stem cells into the peripheral blood. Mononuclear cells were harvested from 10 mL carotid artery blood and then subjected to concentration adjustment to 2×109/L with enough amount of physiological saline. At 4, 3, 2 days before transplantation, 50 mg/kg cyclophosphamide was injected from the tail vein, once a day. On the day of transplantation, mononuclear cells were injected via the tail vein through the use of insulin injection needle. Rats in the model group were given no interventions. Rats in the adjuvant group received equal amounts of physiological and cyclophosphamide.
RESULTS AND CONCLUSION: Titer of serum acetylcholine receptor Ab (AChR Ab titer), CD28, cytotoxic T cell antigen-4, B7.1 and B7.2 expression were significantly greater in the stem cell transplantation group than those in the model group (P < 0.01). AChR Ab titer was positively correlated with cytotoxic T cell antigen-4 in the model and stem cell transplantation groups (r = 0.236, P = 0.001 and r = 0.215, P =0.013). The Lennon scores were significantly lower in the stem cell transplantation group than in the model group (P < 0.01). These findings suggest that stem cell transplantation can regulate organism’s immune system by inhibiting CD28-B7 co-stimulatory pathway.

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