Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (32): 5936-5940.doi: 10.3969/j.issn.2095-4344.2012.32.009

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Proliferation and differentiation potential of bone marrow-derived mesenchymal stem cells in adult patients with aplastic anemia versus in healthy adults

Cheng Mei, Zhang Hao, Tao Yan-ling, Cheng Huan-chen, Wang Zhi-guo, Chen Bo, Xiao Liang, Xia Guo-qiang, Qiu Lin, Zhan Zhao-min, Zhang Bo-long, Ma Jun   

  1. Institute of Hematology & Oncology, Harbin First Hospital, Harbin 150010, Heilongjiang Province, China
  • Received:2011-12-04 Revised:2011-12-04 Online:2012-08-05 Published:2012-08-05
  • Contact: Zhang Hao, Associate chief physician, Institute of Hematology & Oncology, Harbin First Hospital, Harbin 150010, Heilongjiang Province, China gx-zhanghao@126.com
  • About author:Cheng Mei★, Master, Assistant researcher, Institute of Hematology & Oncology, Harbin First Hospital, Harbin 150010, Heilongjiang Province, China chengmei0451@126.com

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Abstract:

BACKGROUND: Bone marrow-derived mesenchymal stem cells (BMSCs) have adipogenic and osteogenic differentiation potential. BMSCs in patients with aplastic anemia (AA) may suffer from adipogenic and osteogenic differentiation abnormality.
OBJECTIVE: To compare the proliferation and differentiation potential of BMSCs in AA patients versus in healthy adults.
METHODS: AA group included five adult patients who received treatment in the Institute of Hematology & Oncology, Harbin First Hospital. Control group consisted of five healthy adults. BMSCs were isolated by whole bone marrow culture method.
RESULTS AND CONCLUSION: BMSCs were isolated and purified successfully and effectively. Morphology and immunophenotype of BMSCs from AA group showed no significant difference compared with control group. Two groups were all positive for MSC-related antigens, such as CD73, CD90, CD105, but negative for CD34, CD45, and HLA-DR. Compared with the control group, BMSC proliferation was significantly lower (P < 0.05), the frequency of adipocyte differentiation was significantly higher (P < 0.05), PPARγ mRNA and FABP4 mRNA expression was significantly higher (P < 0.05), the frequency of osteoblast differentiation was significantly lower (P < 0.05), and ALP mRNA and BGLAP mRNA expression was significantly decreased (P < 0.05) in the AA group. These findings suggest that BMSCs from AA patients exhibit unbalanced differentiation potential of osteogenesis and adipocyte.

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