Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (32): 5931-5935.doi: 10.3969/j.issn.2095-4344.2012.32.008

Previous Articles     Next Articles

Effects of recombinant human erythropoietin on migration and adhesion of human mesenchymal stem cells

Lin Hai-hong1, Luo Xin-ping2, Shi Hai-ming2, Gong Hui1   

  1. 1Department of Cardiology, Jinshan Hospital, Fudan University, Shanghai 201508, China;
    2Department of Cardiology, Huashan Hospital, Fudan University, Shanghai 200040, China
  • Received:2012-03-01 Revised:2012-05-05 Online:2012-08-05 Published:2012-08-05
  • Contact: Gong Hui, Studying for doctorate, Associate chief physician, Department of Cardiology, Jinshan Hospital, Fudan University, Shanghai 201508, China
  • About author:Lin Hai-hong☆, M.D., Attending physician, Department of Cardiology, Jinshan Hospital, Fudan University, Shanghai 201508, China linhh99@hotmail.com

PDF

367

Abstract:

BACKGROUND: Previous studies have demonstrated that erythropoietin can promote the proliferation, differentiation and adhesion of endothelial progenitor cells.
OBJECTIVE: To investigate the effects and related cell signaling pathways of recombinant human erythropoietin on migration and adhesion of human mesenchymal stem cells.
METHODS: After being cultured in vitro, passage 6 human mesenchymal stem cells were treated with recombinant human erythropoietin. Recombinant human erythropoietin, phosphorylation of ERK 1/2, p38MAPK and PI3K/Akt were detected by western blot. After 24 hours of pretreatment with 1U/mL recombinant human erythropoietin, cell migration assay was performed in Transwell chambers, and adhesion assay was performed by plastic dishes.
RESULTS AND CONCLUSION: Recombinant human erythropoietin could increase phosphorylation of PI3K/Akt pathway of human MSCs, but reduce phosphorylation of p38MAPK. It had no obvious effect on ERK1/2 pathway and total proteins of Akt and p38MAPK. The number of cells crossing the filter was significantly increased in the recombinant human erythropoietin group (P < 0.01). This effect was completely inhibited by concomitant incubation with Ly294002. After treated with recombinant human erythropoietin, adherent cells increased significantly (P < 0.01). Neither Ly294002 nor anisomycin could inhibit this effect. Recombinant human erythropoietin could increase migratory and adhesive capacities of human mesenchymal stem cells. Activation of PI3K/Akt pathway was involved in the effect of recombinant human erythropoietin on cell migration. The effect of rhEPO on adhesion capacity had nothing to do with PI3K/Akt and p38MAPK pathway.

CLC Number: