Abstract:

BACKGROUND: Arginine-glycine-aspartic acid (RGD) has strong adhesion, and can graft with biological scaffold materials without changing surface physical and chemical properties of the materials.
OBJECTIVE: To observe the effect of surface modification with RGD polypeptides on the adhesion of bone marrow stem cells (BMSCs) to biological scaffolds.
METHODS: Cells and cellular components were removed from porcine aortic valve by using trypsin and hyposmosis TritonX-100 methods, and then modified with tyrosine-glycine-arginine-glycine-aspartic acid-serine-proline peptides. Decellularized valve scaffolds were divided into different groups according to the concentration of RGD peptides (0.5, 1.0, 1.5, 2.0 g/L), reaction times (4, 8, 12, 24 hours) and reaction pH values (7.0, 7.4, 8.0).
RESULTS AND CONCLUSION: The results of ninhydrin reaction showed cells were prone to attach the decellularized valve scaffolds coupled with RGD peptides. Optimum reaction conditions were at room temperature, the concentration of RGD peptides was 1.5 g/L, PH was 7.4, and sustained oscillation for 12 hours. It is indicated that decellularized valve scaffold modified with tyrosine-glycine-arginine-glycine-aspartic acid-serine-proline peptides can significantly improve the BMSCs adhesion.