Abstract:

BACKGROUND: Magnesium alloy is a new potential medical biodegradable metal material has been obtained more and more attention; in vitro pure magnesium alloy leaching liquor has hemolysis.
OBJECTIVE: To evaluate the relationship between pure magnesium alloy leaching liquor and its haemolyticus.
METHODS: Magnalium alloy leaching liquor was diluted with normal sodium. Haemolysis test was preformed with the improved method which mentioned in the “Haemolysis Test”, the 13th part of “Experimental Methods for the Biological Evaluation of Medical Silicone Materials”. The absorbance value was measured, the haemolyticus was calculated, and the concentration of sodium, magnesium ion in dilution solution and the pH value was detected.
RESULTS AND CONCLUSION: Haemolyticus of the magnesium alloy leaching liquor with 100%, 50%, 25%, 10%, 5%, 2.5% and 1% dilution solution was 65.3%, 66.7%, 33.8%, 11.8%, 6.2%, 0.3% and -0.3%, respectively. With the increased concentration of dilution solution, the concentration of magnesium ion was decreased, but the concentration of sodium ion was not changed obviously. There was no difference in the pH value of 100%, 50%, 25%, 10%, 5% and 2.5% dilution solution (P > 0.05), and all the pH value in these group was higher than that in 1% dilution solution group (P < 0.05). High pH value and ionic concentration in magnalium leaching liquor were the main factors of haemolysis test in vitro.