Table of Content

17 June 2012, Volume 16 Issue 25 Previous Issue    Next Issue

For Selected:

Download Citations EndNote Reference Manager ProCite BibTeX RefWorks

Toggle Thumbnails

Select

Osteogenetic effect of Bio-Oss bone powder combined with bone marrow stromal stem cells Li Xiang, Huang Dai-ying, Chen Song-ling, Zhu Shuang-xi 2012, 16 (25):  4561-4565.  doi: 10.3969/j.issn.1673-8225.2012.25.001 Abstract ( 307 )   PDF (514KB) ( 360 )   Save BACKGROUND: The use of bone tissue engineering in maxillary sinus augmentation is a research hotspot in oral implantology at present. OBJECTIVE: To investigate the osteogenic effect of bone tissue engineering on maxillary sinus floor augmentation with simultaneous dental implant. METHODS: The bone marrow stromal stem cells were isolated from dogs and cultured in vitro. Then, the cells were cultured with Bio-Oss bone powder and induced to differentiate to osteoblasts. Twelve healthy adult dogs were subjected to bilateral maxillary sinus elevation with simultaneous dental implant. One side was implanted with the compound of bone marrow stromal stem cells and Bio-Oss bone power, and the other side was implanted with Bio-Oss bone power alone as controls. RESULTS AND CONCLUSION: Gross observation showed that the implants were stable and not loose, as well as maxillary sinus mucosa was intact. New bone at the experimental side formed earlier and increased in bone mass, while new bone at the control side formed more slowly. X-ray examination showed that new bone at the experimental side was dense and combined tightly with implants. Biomechanical testing indicated that the pull-out force was increased with time, and there were significant differences between the experimental and control sides at weeks 12 and 24 (P < 0.05). Histomorphological analysis exhibited that new bone area was increased gradually, and there were significant differences between the experimental and control sides at weeks 12 and 24 (P < 0.05). These findings suggest that the tissue engineered bone can obtain a good effect on maxillary sinus augmentation with simultaneous dental implant. Related Articles | Metrics

Select

Effect of bone matrix gelatin/true bone ceramic on the osteogenic potential of bone marrow mesenchymal stem cells Liu Xi-min, Tian Zhi-feng, Zhang Xiao-ling, Wang Feng-gang 2012, 16 (25):  4566-4570.  doi: 10.3969/j.issn.1673-8225.2012.25.002 Abstract ( 166 )   PDF (541KB) ( 337 )   Save BACKGROUND: Previous studies have found that simply bone matrix gelatin (BMG) material compound with bone marrow mesenchymal stem cells (BMSCs) has a good osteogenic potential for bone defects with better effects, but simply BMG cannot be used to repair bone defects in load-bearing area because of rapid degradation speed and bad hardness. OBJECTIVE: To observe the changes in the osteogenic potential of BMSCs co-cultured with BMG/true bone ceramic (BMG/TBC). METHODS: BMG and TBC were used to prepare recombinant bone that was co-cultured with BMSCs for 48 hours in vitro. Osteogenic potential of the recombinant bone was detected by Alizarin bordeaux staining. RESULTS AND CONCLUSION: BMSCs in composited material could adhere, grow and proliferate well after co-cultured with BMG/TBC. There were no changes of the osteogenic potential and cell phenotype. It is indicated that BMG/TBC is easy to combined with BMSCs without affecting osteogenic potential, which shows a good biocompatibility. Related Articles | Metrics

Select

A new type of bone stuff with bone morphogenetic protein is used in vertebroplasty Qian Guang, Yang Wen-cheng, Wang Ming-hai, Dong You-hai 2012, 16 (25):  4571-4575.  doi: 10.3969/j.issn.1673-8225.2012.25.003 Abstract ( 183 )   PDF (561KB) ( 550 )   Save BACKGROUND: Calcium phosphate bone cement (CPC) has good biocompatibility and no disadvantages of polymethyl methacrylate (PMMA). CPC with recombinant human bone morphogenetic protein-2 (rhBMP-2) has a microporous structure, and its clinical value can be improved in percutaneous vertebroplasty. OBJECTIVE: To evaluate the feasibility of injectable CPC and fibrin sealant (FS) combined with rhBMP-2 in vertebroplasty of New Zealand white rabbits to replace PMMA. METHODS: CPC/FS/rhBMP-2 was prepared. Tight muscle pouch model in mice was used to evaluate the osteoinductive activities of the implant materials. Imitation of vertebral plasty was used to observe the biomechanical changes of new composite material and PMMA after their implantation. RESULTS AND CONCLUSION: At 2 and 4 weeks of CPC/FS/rhBMP-2 implantation, alkaline phosphatase levels were the highest. At 4 weeks of CPC/FS/rhBMP-2 implantation, new bone formation and chondrocyte maturation could be seen, and the compressive strength and torsion strength were obviously lower than those of the normal vertebral and PMMA implantation (P < 0.05). After 8 weeks of implantation, part of the CPC/FS/rhBMP-2 cement was degraded with some increases in compressive strength and torsion strength, and the torsion strength was similar with that of the normal vertebral, but was lower than that of PMMA implantation (P < 0.05). Micro CT showed that the new bone was plenty and its formation was in the early stage, and there was no material absorption or surrounding bone ingrowth could be seen in PMMA. It is indicated that good bone induction and bone conduction can be obtained after CPC/FS/rhBMP-2 implantation, and the degradation of CPC/FS/rhBMP-2 can synchronize with new bone formation to achieve normal bone healing. CPC/FS/rhBMP-2 is expected to replace PMMA in vertebral plasty. Related Articles | Metrics

Select

Calcium phosphate bone cement combined with autologous vascularized periosteum in the repair of segmental bone defects Zhang Jun-guo, Pan Zhao-hui, Zhao Yu-xiang 2012, 16 (25):  4576-4580.  doi: 10.3969/j.issn.1673-8225.2012.25.004 Abstract ( 190 )   PDF (479KB) ( 357 )   Save BACKGROUND: Calcium phosphate bone cement (CPC) is a new-type bone substitute material, but it has slow degradation and poor mechanical properties. OBJECTIVE: To investigate the feasibility of CPC combined with autologous vascularized periosteum in repairing segmental bone defects. METHODS: Totally 72 New Zealand rabbits were randomly divided into four groups: non-CPC groups with/without periosteum retention and CPC groups with/without periosteum retention. The specimens were tested by general, radiology, histology and biomechanics tests at weeks 4, 12 and 24 after repair. RESULTS AND CONCLUSION: Non-CPC groups: the callus was found at week 12, as well as bone sclerosis and medullary cavity closure was seen at week 24 in non-periosteum retention group; a spot of calluses was found at week 4, and callus were increased at week 12, as well as oblique new bone was formed at week 24 in periosteum retention group. CPC groups: in the non-periosteum retention group, boundaries between material and bone end were found obviously at week 4, and the fuzzy decomposition was seen at week 12, besides, materials and bone end formed bony connection at week 24; in the periosteum retention group, no degradation in materials was found at week 4 and material degradation were seen at week 12, moreover, material absorption was more and the medullary cavity was recanalized at week 24. CPC groups were performed three point bending biomechanics test at week 24, and the results showed that the maximum load of the non-periosteum retention group was lower than that of the periosteum retention group (P < 0.05). These findings suggest that CPC can promote bone growth effectively, and autologous periosteum is helpful to calcium absorption and promotes bone formation. Related Articles | Metrics

Select

Bone cement mixed with different antibiotics using different methods Wei Bo, Wang Chen, Li He 2012, 16 (25):  4581-4585.  doi: 10.3969/j.issn.1673-8225.2012.25.005 Abstract ( 283 )   PDF (420KB) ( 561 )   Save BACKGROUND: In artificial joint replacement surgery, which antibiotic mixed with bone cement can play an effective prevention against postoperative infection is still controversial. OBJECTIVE: To study the release characteristics of antibiotic in animals and the mechanical properties of bone cement based on the observation of bone cement mixed with different antibiotics using different mixture methods. METHODS: Thirty-six white rabbits were randomly divided into six groups: gentamicin group Ⅰ, vancomycin group Ⅰ, cefuroxime sodium group Ⅰ, gentamicin group Ⅱ, vancomycin group Ⅱ, cefuroxime sodium group Ⅱ. In the former three groups, 2 g of gentamicin sulfate, 1 g of vancomycin and 1.5 g of cefuroxime sodium were added into 40 g of bone cement at different phases, respectively, to prepare antibiotic-loaded bone cements that were then implanted into the rabbits. In the latter three groups, 2 g of gentamicin sulfate, 1 g of vancomycin and 1.5 g of cefuroxime sodium were respectively added before the solid-phase and liquid-phase mixture of 40 g bone cement. RESULTS AND CONCLUSION: The average release time of three antibiotics was over 31 days in rabbits. The elution volume of antibiotics in the former three groups was higher than that in the latter three groups (P < 0.05). The elution volume in the vancomycin group Ⅰ was higher than that in the other groups (P < 0.05). The mechanical properties of antibiotic-loaded bone cements in each group were better than international standard ISO 5833, and there was no significant difference among the groups. These findings indicate that antibiotics can release from bone cement effectively; bone cements containing 1-2 g of antibiotics cannot affect the mechanical strength of bone cements; the elution effect of vancomycin is better; the method to add antibiotics after the solid-phase and liquid-phase bone cement mixture is more conducive to the release of antibiotics. Related Articles | Metrics

Select

Different bone graft materials induce the lumbar intertransverse process fusion in rabbits Sun Jun-chao, Xiao Rong-chi, Ou Jun, Jiang Yu-min, Sun Xu-hua 2012, 16 (25):  4586-4590.  doi: 10.3969/j.issn.1673-8225.2012.25.006 Abstract ( 242 )   PDF (563KB) ( 325 )   Save BACKGROUND: Chemical bonding can be formed between the material interface and human bone after implantation of bioactive glass as bone graft into human body. Bioactive glass can induce bone repair and regeneration, but its poor bending strength may lead to stress shielding and failure of bone repair. OBJECTIVE: To observe the effects of different bone graft materials on lumbar intertransverse fusion of rabbits, and to investigate their feasibilities to replace autologous bone in lumbar intertransverse fusion. METHODS: Twenty-eight healthy male adult New Zealand white rabbits were randomly divided into four groups: chitosan/ calcium-silicate group, calcium-silicate group, chitosan group and autologous ilium group. Lumbar posterolateral fusion was performed in all rabbits to place the implant materials into both sides of L4/5 intertransverse. RESULTS AND CONCLUSION: At 12 weeks after surgery, bone fusion was found in seven rabbits of the chitosan/calcium- silicate group, four rabbits of the calcium-silicate and chitosan group, and five rabbits of the autologous ilium group. Histological observation showed that the chitosan/calcium-silicate could obviously promote the degradation and osteogenesis. Biomechanical test showed that tensile strength of the chitosan/calcium-silicate group was superior to the other groups (P < 0.05). The results indicated that the three materials can replace autologous bone in lumbar intertransverse fusion, and chitosan/calcium-silicate can promote the process and improve the biomechanical strength of lumbar intertransverse fusion. Related Articles | Metrics

Select

Construction of the scaffold composite of bone marrow mesenchymal stem cells and Schwann cells after NgR gene silencing Wang Dong, Zhang Jian-jun, Yang Wei-shan 2012, 16 (25):  4591-4595.  doi: 10.3969/j.issn.1673-8225.2012.25.007 Abstract ( 174 )   PDF (533KB) ( 381 )   Save BACKGROUND: Combined cell transplantation, NgR gene silencing and poly-lactic acid-glycolic acid (PLGA) scaffolds have become the research focus of spinal cord injury repair in recent years. OBJECTIVE: To investigate the feasibility of the growth and differentiation of bone marrow mesenchymal stem cells (BMSCs)/Schwann cells in poly (lactic-co-glycolic acid) (PLGA) membrane after NgR gene silencing. METHODS: After BMSCs and Schwann cells were isolated, purified and amplified, the two kinds of cells were transfected by small interfering RNA to silence NgR gene expression. The NgR gene/protein expressions in the two kinds of cells before and after transfection were detected by reverse transcription-PCR and Western blot methods. Under serum conditions, the cells were divided into BMSCs group, Schwann cells group and combination group (all untransfected cells as controls, six groups in total). Then, the cells were inoculated onto the PLGA membrane, and their adhesion, growth and differentiation were observed. RESULTS AND CONCLUSION: The NgR gene and protein expressions of the experimental group were significantly decreased after small interfering RNA transfection (P < 0.05). Compared with the untransfected cells groups after culture, a large number of implanted cells adhered and grew on the PLGA membrane in NgR gene silencing groups. These findings suggest that NgR gene silencing can promote BMSCs/Schwann cells adhesion, growth and differentiation. Related Articles | Metrics

Select

Antibacterial effects of nano zinc oxide on Candida albicans He Lin-hai, Meng Song, Liu Miao-miao, Zhou Di-shun, Li Shan, Luo Mei, Ma Xing-ming, Wang Xue-mei 2012, 16 (25):  4596-4600.  doi: 10.3969/j.issn.1673-8225.2012.25.008 Abstract ( 382 )   PDF (461KB) ( 902 )   Save BACKGROUND: As an inorganic antibacterial agent, nano zinc oxide has different antibacterial mechanisms from organic fungicides. OBJECTIVE: To explore the antibacterial effects of nano zinc oxide on the fungus, such as Candida albicans. METHODS: Nano zinc oxide and zinc oxide were prepared as the suspension with concentrations of 100, 75, 50, 25 and 10 g/L respectively. Normal saline served as a negative control. 30 μL suspension with different concentrations was acted on Candida albicans by using Kirby-Bauer disk diffusion method. The culture plate was cultured at a 37 ℃ incubator for 24 hours. The size of inhibition zone was observed and measured. RESULTS AND CONCLUSION: With the increased concentration of nano zinc oxide, the antibacterial effect on Candida albicans was enhanced gradually, and the diameter of inhibition zone was enhanced. There were no obvious antibacterial effects of zinc oxide on Candida albicans. These findings suggest that nano zinc oxide can inhibit the growth of Candida albicans. Related Articles | Metrics

Select

Modified nano-silica by two kinds of coupling agents influences the mechanical properties of resin base Xiao Yue, Liu Bin, Wang Jian-ping, Yu Ting-ting, Wang Jin-gang 2012, 16 (25):  4601-4604.  doi: 10.3969/j.issn.1673-8225.2012.25.009 Abstract ( 190 )   PDF (398KB) ( 327 )   Save BACKGROUND: Polymethyl methacryl (PMMA) is widely used based on its good biocompatibility, good color and ease of molding. However, this material is easy to be fractured because of its poor toughness and hardness. OBJECTIVE: To study the changes of bending strength, flexural modulus and hardness of PMMA materials induced by modified nano-SiO2 particles which are modified with two kinds of coupling agents. METHODS: SiO2/PMMA nanocomposites were prepared with 3% nano-SiO2 particles modified by two kinds of coupling agents (KH-570 and KH-502) and PMMA powders. There were four groups, PMMA group, SiO2/PMMA group, KH-570-SiO2/PMMA group and KH-502-SiO2/PMMA group. RESULTS AND CONCLUSION: The bending strength, flexural modulus and harnesses of the SiO2/PMMA group and the PMMA group had no significant difference (P > 0.05), while the bending strength, flexural modulus and hardness of modified-SiO2/PMMA were obviously increased compared with PMMA group (P < 0.05). The KH-502-SiO2/PMMA had the best bending strength, flexural modulus and harnesses among these groups (P < 0.05). It is indicated that the SiO2/PMMA nanocomposites prepared by adding modified-SiO2 particles into PMMA have better bending strength, flexural modulus and harnesses. Performance improvement is more obvious in KH-502-SiO2/PMMA. Related Articles | Metrics

Select

One-pot synthesis and formation mechanism of water-soluble PbS/gelatin micro-nano biocomposite Wang Jun, Li You-qun, Mao Xin-jun, Tang Shi-hua 2012, 16 (25):  4605-4610.  doi: 10.3969/j.issn.1673-8225.2012.25.010 Abstract ( 237 )   PDF (572KB) ( 409 )   Save BACKGROUND: Studieds about the synthesis and formation mechanism of bionanocomposites can provide theoretical basis for the design and wide application of inorganic/organic composites in biomedical area. OBJECTIVE: To synthesize water-soluble PbS/gelatin micro-nano biocomposites at pH value of 7.40, and to study the intergration mechanism of PbS particles in the macromolecules of gelatin. METHODS: The water-soluble PbS/gelatin micro-nano biocomposites were synthesized via one-pot chemical route involving the reaction of Pb2+ with S2− in gelatin aqueous solution at pH value of 7.40. The particle shape, size and spectral quality of samples were characterized by scanning electron microscopy, dynamic light scattering, UV-vis spectroscopy and Fourier transform infrared spectroscopy. RESULTS AND CONCLUSION: Scanning electron microscopy images showed that the morphologies of PbS particles were mainly irregular polyhedrons and olive-shape in the lower and higher concentration of Pb2+, respectively. Dynamic light scattering results indicated that the average particle sizes of PbS/gelatin micro-nano biocomposites were 75, 91 and 109 nm in the presence of 2.0×10-5, 8.0×10-5 and 2.0×10-4 mol/L of Pb2+, respectively. And they showed an obvious quantum confinement effect. Based on the experimental results, the possible formation mechanism is proposed as follows: there is a main interaction between the Pb2+ ions and the ketonic oxygen on the peptide chain of gelatin macromolecules, which causes the gelatin macromolecules conformation changed from α-helix to β-sheet for the oriented nucleation of PbS; and then the S2− is combined with Pb2+ to form PbS nanoparticles. The PbS/gelatin micro-nano biocomposites showed a better stability in aqueous solution, owing to gealtin polypeptide chain coating on the surface of PbS nanoparticles. Related Articles | Metrics

Select

Effect of FiltekTM Z350 flowable nano-composite resin on the repair of early pit and fissure caries with minimally invasive dentistry technique Gu Yuan-ping, Qin Ming-qun, Mao Jun-wu, Liu Li, Jiang Ying-quan 2012, 16 (25):  4611-4615.  doi: 10.3969/j.issn.1673-8225.2012.25.011 Abstract ( 243 )   PDF (456KB) ( 587 )   Save BACKGROUND: The traditional treatment for early pit and fissure caries on permanent teeth is to remove caries tissue and prepare the cavity according to class Ⅰ, but which will cause the excessive loss of tooth substance. OBJECTIVE: To review the clinical effect of flowable nano-composite resin on repairing early pit and fissure caries with minimally invasive dentistry technique. METHODS: A total of 206 permanent molars with occlusal pit and fissure caries in 167 patients who aged from 14 to 30 years were included. After unilateral cariated tissue had been removed, the cavity was treated with Adper Single Bond 2 total-etching adhesive method, and the whole cavity was filled with Filtek TM Z350 flowable nano-composite resin with minimally invasive dentistry technique and as experimental group. The opposite or adjacent teeth with similar caries in the same patients were treated with Filtek TM Z350 nano-resin as control group. RESULTS AND CONCLUSION: All the patients were followed up for 2 years. In the experimental group, the Filtek TM Z350 flowable nano-composite resin exhibited higher retention rate, better marginal integrity and lower marginal discoloration than those of the control group (P < 0.05). These findings suggest that Filtek TM Z350 flowable nano-composite resin can effectively repair early pit and fissure caries with minimally invasive dentistry technique. Related Articles | Metrics

Select

Biocompatibility of acellular dermal matrix with human adipose-derived stem cells Luo Zhi-jun, Li Hong-mian, Wang He-geng, Liu Da-lie, Nan Hua 2012, 16 (25):  4616-4621.  doi: 10.3969/j.issn.1673-8225.2012.25.012 Abstract ( 168 )   PDF (562KB) ( 464 )   Save BACKGROUND: Acellular dermal matrix has a good biocompatibility and the inductive effect on tissues or cells. OBJECTIVE: To evaluate the biocompatibility of acellular dermal matrix with human adipose-derived stem cells (ADSCs). METHODS: Human ADSCs were isolated from the subcutaneous adipose tissue of healthy adult after liposuction, and primary culture and subculture of human ADSCs were conducted. Human ADSCs of passage 3 were co-cultured with the acellular dermal matrix in vitro for 3-7 days. Cell growth, proliferation and adhesion on the scaffolds were observed under inverted phase contrast microscope and scanning electron microscope, and the adhesion rate was analyzed. XTT-colorimetry was used to observe the cell growth and proliferation. RESULTS AND CONCLUSION: Human ADSCs distributed evenly on the acellular dermal matrix scaffold. The cells expanded and adhered on the scaffold within 24 hours. The cells showed extensional deformation at 2-3 days, and the cell shape was spindle in main and mesh arranged. Cells on the scaffold were gradually increased as time went on. The average adherens rate was 95.03% of ADSCs co-cultured with acellular dermal matrix scaffold, and the cell growth and proliferation were normal. It is indicated that ADSCs can well attached to acellular dermal matrix scafold and normally grow and proliferate on the scaffolds. Acellular dermal matrix scaffold has a good biocompatibility with ADSCs. Related Articles | Metrics

Select

Promoted effect of surface modification with arginine-glycine-aspartic acid polypeptides on the adhesion of bone marrow stem cells to biological scaffolds Li Wei, Xu Geng-qiang, Zhou Hua-song, Han Hai-xia 2012, 16 (25):  4627-4631.  doi: 10.3969/j.issn.1673-8225.2012.25.014 Abstract ( 280 )   PDF (533KB) ( 334 )   Save BACKGROUND: Arginine-glycine-aspartic acid (RGD) has strong adhesion, and can graft with biological scaffold materials without changing surface physical and chemical properties of the materials. OBJECTIVE: To observe the effect of surface modification with RGD polypeptides on the adhesion of bone marrow stem cells (BMSCs) to biological scaffolds. METHODS: Cells and cellular components were removed from porcine aortic valve by using trypsin and hyposmosis TritonX-100 methods, and then modified with tyrosine-glycine-arginine-glycine-aspartic acid-serine-proline peptides. Decellularized valve scaffolds were divided into different groups according to the concentration of RGD peptides (0.5, 1.0, 1.5, 2.0 g/L), reaction times (4, 8, 12, 24 hours) and reaction pH values (7.0, 7.4, 8.0). RESULTS AND CONCLUSION: The results of ninhydrin reaction showed cells were prone to attach the decellularized valve scaffolds coupled with RGD peptides. Optimum reaction conditions were at room temperature, the concentration of RGD peptides was 1.5 g/L, PH was 7.4, and sustained oscillation for 12 hours. It is indicated that decellularized valve scaffold modified with tyrosine-glycine-arginine-glycine-aspartic acid-serine-proline peptides can significantly improve the BMSCs adhesion. Related Articles | Metrics

Select

Haemolyticus of manalium alloy and influencing factors Chen Xu-qiong, Yin Qing-shui, Zhang Yu, Wang Zhi-yun 2012, 16 (25):  4632-4636.  doi: R318 Abstract ( 293 )   PDF (423KB) ( 538 )   Save BACKGROUND: Magnesium alloy is a new potential medical biodegradable metal material has been obtained more and more attention; in vitro pure magnesium alloy leaching liquor has hemolysis. OBJECTIVE: To evaluate the relationship between pure magnesium alloy leaching liquor and its haemolyticus. METHODS: Magnalium alloy leaching liquor was diluted with normal sodium. Haemolysis test was preformed with the improved method which mentioned in the “Haemolysis Test”, the 13th part of “Experimental Methods for the Biological Evaluation of Medical Silicone Materials”. The absorbance value was measured, the haemolyticus was calculated, and the concentration of sodium, magnesium ion in dilution solution and the pH value was detected. RESULTS AND CONCLUSION: Haemolyticus of the magnesium alloy leaching liquor with 100%, 50%, 25%, 10%, 5%, 2.5% and 1% dilution solution was 65.3%, 66.7%, 33.8%, 11.8%, 6.2%, 0.3% and -0.3%, respectively. With the increased concentration of dilution solution, the concentration of magnesium ion was decreased, but the concentration of sodium ion was not changed obviously. There was no difference in the pH value of 100%, 50%, 25%, 10%, 5% and 2.5% dilution solution (P > 0.05), and all the pH value in these group was higher than that in 1% dilution solution group (P < 0.05). High pH value and ionic concentration in magnalium leaching liquor were the main factors of haemolysis test in vitro. Related Articles | Metrics

Select

Comparison of material mechanics and histocompatibility between collagen/hyaluronic acid membrane and gelatin sponge scaffold Xiao Rong-dong, Weng Guo-xing 2012, 16 (25):  4637-4643.  doi: 10.3969/j.issn.1673-8225.2012.25.016 Abstract ( 327 )   PDF (620KB) ( 582 )   Save BACKGROUND: Our previous studies have confirmed that collagen/hyaluronic acid membrane has good mechanical properties and histocompatibility. OBJECTIVE: To explore the biological properties of collagen/hyaluronic acid membrane and gelatin sponge scaffold. METHODS: The collagen/hyaluronic acid membrane was constructed by compound cross linking method. The mechanical property of the collagen/hyaluronic acid membrane and gelatin sponge scaffold was detected. The scaffold materials were implanted subcutaneously in rabbits; the in vivo degradation and mechanical properties were evaluated at 2, 4, 6, 8 and 12 weeks after implantation. RESULTS AND CONCLUSION: Collagen/hyaluronic acid membrane was constructed successfully. Collagen/hyaluronic acid membrane had a better toughness and tensile strength while the mechanical property of gelatin sponge was not ideal. The in vivo degradation of the two materials was compatible with the tissue reactions of biomaterials. Complete degradation of the collagen/hyaluronic acid membrane could be done within 12 weeks, while the gelatin sponge within 6 weeks. The adhesion rate between the collagen/hyaluronic acid membrane and smooth muscle cells was high; the cell proliferation and the metabolic status were good. The adhesion rate and proliferation rate of the gelatin sponge was relatively low. These findings indicate that gelatin sponge has better biological properties. Related Articles | Metrics

Select

Biocompatibility and safety of inferior vena cava filter implantation in preventing pulmonary embolism Sun Yu-gui, Huang Bao-tang, Yu Jin 2012, 16 (25):  4644-4648.  doi: 10.3969/j.issn.1673-8225.2012.25.017 Abstract ( 296 )   PDF (467KB) ( 512 )   Save BACKGROUND: Inferior vena cava filter can prevent pulmonary embolism in clinical practice. OBJECTIVE: To explore the biocompatibility, safety, surgical indications, and causes and preventive measures of related complications of inferior vena cava filter implantation in preventing pulmonary embolism. METHODS: A retrospective analysis was performed in 96 patients in the People’s Hospital of Guilin from 2001 to 2011 receiving vena cava filter implantation for treatment of deep venous thrombosis. RESULTS AND CONCLUSION: Fifty-five patients were implanted with permanent filter, 23 cases were implanted with recyclable filter, and 18 patients were with temporary filter. All the patients were followed up for 8 months to 7 years. Pulmonary embolism occurred in one patient, filter tilting happened in two cases, and slight host response could be seen in one case, all of the above did not influence the treatment. It is indicated that inferior vena cava filter can efficiently prevent pulmonary embolism in clinical practice with good biocompatibility and safety. Related Articles | Metrics

Select

Changes in surface reaction layers during titanium casting using different investments Liu Jie, Gu Xiao-ming, Chen Xiang-tao, Fan Jin-jie, Wang Jing 2012, 16 (25):  4649-4653.  doi: 10.3969/j.issn.1673-8225.2012.25.018 Abstract ( 313 )   PDF (462KB) ( 405 )   Save BACKGROUND: So far, comparative studies about the application of magnesium oxide and zirconium oxide-bonded materials in pure titanium casting have not been reported in detail, but phosphate-bonded investment has an obvious stratification on the casting surface reaction layers, and can be used as the control. OBJECTIVE: To study the effects of different investments on the surface reaction layer of cast titanium. METHODS: Phosphate, zirconium oxide and magnesium oxide investments were embedded for pure titanium casting with specification of embedded 10 mm x 10 mm x 1 mm respectively, and the casting surface reaction layers were tested by scanning electron microscope, energy dispersive spectrometer, X-ray diffraction, micro-hardness and metallographic methods. RESULTS AND CONCLUSION: The surface reaction layer thickness of the castings which cast by phosphate, zirconium oxide and magnesium oxide investments in metallographic photos was 70, 45, and 12 μm, respectively. The hardness value of these three species castings was obviously reduced 100, 60 and 40 μm from the surface respectively; from this place to the internal titanium cast, their hardness value changed stably. Major element Al, Si, O content in reaction layer was decreased gradually as the depth of reaction layer increased. Compared with zirconium oxide and phosphate investments, the reaction between magnesium oxide investment and titanium casting was slighter, and reaction layers were less, moreover, there was less pollution in pure titanium casting. These findings suggest that magnesium oxide investment is the most ideal investment for pure titanium casting. Related Articles | Metrics

Select

Non-isothermal crystallization kinetics of degradable copolymer based on epsilon-caprolactone and D, L-lactide Yang Li-qun, Yang Dan, Li Jian-xin, Guan Yan-min, Li Miao 2012, 16 (25):  4654-4658.  doi: 10.3969/j.issn.1673-8225.2012.25.019 Abstract ( 229 )   PDF (580KB) ( 394 )   Save BACKGROUND: Copolymers of ε-caprolactone and D, L-lactide (PCDLA) has been studied intensely for their good mechanical properties, biodegradability and biocompatibility. However, the study on non-isothermal crystallization kinetics of PCDLA has attracted less attention. OBJECTIVE: To investigate the non-isothermal crystallization kinetics of PCDLA. METHODS: PCDLA with a CL/DLLA molar ratio of 90:10 was prepared by ring-opening polymerization and differential scanning calorimetry was employed to study the non-isothermal crystallization kinetics of PCDLA. The kinetics parameters were analyzed by Avrami, Jeziorny and Mo methods. RESULTS AND CONCLUSION: The crystallization peak temperatures of PCDLA decreased with increasing cooling rate. In the process of non-isothermal crystallization, the value of Avrami index was greater than 3 for PCDLA, which demonstrates a three-dimensional spherulitic growth on homogeneous nuclei. The Jeziorny method of the corrected Avrami theories and Mo method can describe non-isothermal crystallization kinetics of PCDLA very well. Related Articles | Metrics

Select

Preparation of elemene transdermal gel and its skin penetration in vitro Liu Xing-yan, Liu Hong, Fan Hai-zhu, Lin Jing, Zeng Zhao-wu, He Zhi-wei 2012, 16 (25):  4659-4662.  doi: 10.3969/j.issn.1673-8225.2012.25.020 Abstract ( 272 )   PDF (405KB) ( 482 )   Save BACKGROUND: Effect of choosing a suitable carrier material on elemene transdermal properties is the problem of elemene transdermal preparation and need to be resolved first. OBJECTIVE: To prepare an elemene transdermal gel and to investigate its skin penetration in vitro. METHODS: An elemene transdermal gel was prepared by using hydrophilic polymer polyvinyl alcohol and sodium carboxymethyl cellulose each with good biocompatibility. In the experimental installation for skin penetration in vitro, the mouse dorsal skin was isolated as a barrier for percutaneous penetration experiments. Percutaneous infiltration quantity of elemene was detected by high resolution gas chromatography. RESULTS AND CONCLUSION: The ratio of polyvinyl alcohol and scarboxymethyl cellulose showed no significant effect on the transdermal ability of elemene. But their concentration in the gel matrix had a certain influence on the transdermal ability of elemene, and 30% content (total content of polyvinyl alcohol and sodium carboxymethyl cellulose) was optimal. These findings suggest that this kind of transdermal gel can be used as a good carrier for elemene. Related Articles | Metrics

Select

Preparation and in vitro detection of osteogenic growth peptide chitosan-alginate microspheres Ma Shu-qiang, Peng Jian-qiang, Li Zhong-tan, Li Yu-hong, Wu Wei-dong, Lin Qi-sheng, Wang Kun-zheng 2012, 16 (25):  4663-4666.  doi: 10.3969/j.issn.1673-8225.2012.25.021 Abstract ( 229 )   PDF (366KB) ( 536 )   Save BACKGROUND: In vitro injection of osteogenic growth peptide (OGP) can regulate the peripheral blood and proliferation of bone marrow cells to increase bone formation and accelerate fracture healing, but it can not be worldly used for its instability and inconvenience of injection. OBJECTIVE: To prepare the OGP chitosan-alginate microsphere by emulsion crosslinking, and to detect its particle size, drug loading, in vitro release, physical and chemical properties. METHODS: The OGP loaded chitosan-alginic acid microsphere was prepared using an emulsion cross-linking method with glutaral as the cross-linking agent. Microscopy and scanning electron microscopy were used to evaluate the morphological properties and particle size distribution. The drug loading, entrapment efficiency and in vitro drug release kinetics were detected by enzyme-linked immunosorbent assay. RESULTS AND CONCLUSION: The OGP loaded chitosan-alginic acid microspheres were spherical in shape, and showed a asperity surface with many micropores and a higher encapsulation efficiency (> 72%). From the in vitro release experiment, the OGP was controlled to release from the chitosan microsphere over 49 days and the release property of microspheres in vitro was well and the overall release rate was > 85%. It is indicated that the OGP loaded chitosan-alginic acid microsphere that is prepared by emulsion cross-linking method shows a good controlled release property. Related Articles | Metrics

Select

Application of vacuum sealing drainage dressing covered with polyethylene ethanol hydration seaweed salt foam in the Morel-Lavallée lesion complicated with acetabular fractures before internal fixation Li Li-geng, Wu Xiao-bo, Sun Bai-shan, Liang Wei-dong 2012, 16 (25):  4667-4670.  doi: 10.3969/j.issn.1673-8225.2012.25.022 Abstract ( 246 )   PDF (393KB) ( 505 )   Save BACKGROUND: Morel-Lavallée lesions complicated with acetabular fractures are rarely mentioned in literatures, at present how to control the lesion and early fracture fixation has no generally accepted method. OBJECTIVE: To further verify the effect of vacuum sealing drainage covered with polyethylene ethanol hydration seaweed salt foam in the Morel-Lavallée lesion complicated with acetabular fractures. METHODS: Twenty-three patients with Morel-Lavallée lesion complicated with acetabular fractures were divided into two groups. The patients in experimental group received vacuum sealing drainage intervention for the Morel-Lavallée lesion and those in control group received usual debridement before internal fixation of acetabular fractures. The times between the injury and the operation of acetabular fractures of two groups were compared. The clinical results were evaluated by the scores of Merle d’Aubigne and Postel. RESULTS AND CONCLUSION: The scores of Merle d’Aubigne and Postel in the experimental group were significantly higher than those in the control group (P < 0.05). The times between the injury and the operation of acetabular fractures in the experimental and control groups were (20.62±6.01) days and (28.00±8.07) days respectively, and the differences of the time between the two groups were significant (P < 0.05 ). The vacuum sealing drainage can promote the cure of the Morel-Lavallée lesion, then can made it possible for early operation of acetabular fractures, thus can improve the hip joint function for patients of the Morel-Lavallée lesion complicated with acetabular fractures after surgery. Related Articles | Metrics

Select

Design of a transdermal delivery system based on STC12C5S60S2 microcontroller Pan Zhi-xiong, Yu Xue-fei, Peng Da-ming 2012, 16 (25):  4671-4676.  doi: 10.3969/j.issn.1673-8225.2012.25.023 Abstract ( 321 )   PDF (578KB) ( 381 )   Save BACKGROUND: Transdermal drug delivery system provides a convenient and effective way for the delivery of peptides and proteins. OBJECTIVE: To develop a microcontroller-based transdermal drug delivery system, which can provide non-invasive trasdermal drug delivery, maintain the activity of drugs and improve the bioavailability of drugs. METHODS: The microcontroller was used as the control core. Enhancement techniques such as electroporation, iontophoresis and sonophoresis were added into the delivery of proteins. The skin barrier was overcome at different aspects, and the absorption of transdermal drug was increased. Besides, synergistic effect of these three enhancement techniques was achieved via reasonable design based on software and hardware, as well as the efficiency of drug delivery was improved. RESULTS AND CONCLUSION: The developed prototype of transdermal drug delivery system was easy to operate. The non-invasive trasdermal drug delivery can improve patients’ compliance and can be used for trasdermal drug delivery with a variety of drugs by adjusting the treatment parameters, as well as provide the potential for individualized therapy. Related Articles | Metrics

Select

In vitro culture of bone marrow mesenchymal stem cells on silk fibroin/chitsan three-dimensional scaffolds Deng Jiang, She Rong-feng, Huang Wen-liang, Yuan Cheng, Mo Gang 2012, 16 (25):  4677-4681.  doi: 10.3969/j.issn.1673-8225.2012.25.024 Abstract ( 197 )   PDF (592KB) ( 423 )   Save BACKGROUND: Preliminary experiments have confirmed that mixture of silk fibroin and chitosan in a proper ratio can make up their shortcomings to show the good physical and chemical properties and biological characteristics. OBJECTIVE: To observe the growth of bone marrow mesenchymal stem cells (BMSCs) on silk fibroin/chitsan (SF/CS) composite three-dimensional scaffolds. METHODS: Rabbit BMSCs after induction were seeded on SF/CS scaffolds. Cell adhesion rate was detected, and cell growth was observed under inverted microscope and scanning electron microscopy. RESULTS AND CONCLUSION: The cell adhesion rate was increased as time went on. Inverted microscope: BMSCs on the scaffolds could not be seen clearly, the number of cells around the scaffolds was increased as time went on, and the cells extended into the scaffolds. Scanning electron microscopy: the cells grew actively and had normal multiple fissions, granular and filamentous substrates could be seen around the cells, and cell microfilaments were closely connected with the scaffolds; the cells could not only show the attached growth on surfaces of the scaffolds, but could also extend into the scaffolds. It is indicated that the SF/CS composite scaffolds have a good cell biocompatibility. Related Articles | Metrics

Select

Detection of basement membrane components on the surface of acellular porcine cornea Lin Xu-chu, Jin Yan, Hui Yan-nian 2012, 16 (25):  4682-4685.  doi: 10.3969/j.issn.1673-8225.2012.25.025 Abstract ( 377 )   PDF (380KB) ( 449 )   Save BACKGROUND: The previous experiments have suggested that the acellular porcine cornea stroma (APCS) has a good compatibility and can support the growth of keratocytes and skin epithelial cells. OBJECTIVE: To detect whether the important structure for the growth of corneal epithelial cells-basement membrane can be preserved on the surface of the APCS. METHODS: Fluorescent antibody was used to detect the basement membrane component (laminin and collagen Ⅳ) by immunohistochemistry. Fluorescence microscopy was observed whether the basement membrane could be preserved on the surface of the APCS. RESULTS AND CONCLUSION: Immunofluorescence staining showed that laminin and collagen Ⅳ positively expressed on the surfaces of porcine corneal stroma with the same level of fresh normal porcine corneal stroma. The results indicate the APCS can preserve the natural basement membrane component of the cornea which is beneficial to the growth of corneal epithelial cells. Related Articles | Metrics

Select

Surface modification and biocompatibility of medical nickel-titanium shape memory alloys Du Jian, Ding Yuan-fa, Su Xiang-dong, He Li, Hao Wei-chang, Wang Tian-min 2012, 16 (25):  4686-4691.  doi: 10.3969/j.issn.1673-8225.2012.25.026 Abstract ( 301 )   PDF (924KB) ( 995 )   Save BACKGROUND: Near-equiatomic nickel-titanium shape memory alloy can be used as a type of ideal biological implant material based on its deformation rate of 8%, million times in the number of cycles, excellent corrosion resistance and low elastic modulus, low magnetism, and radiopacity. OBJECTIVE: To discuss the surface modification and biocompatibility of the medical near-equiatomic nickel-titanium shape memory alloy. METHODS: A computer-based online search was performed in PubMed database and Wanfang database for literatures from January 1990 to December 2008 related to surface chemical treatment and chemical modification of medical nickel-titanium shape memory alloy and dissolvability of nickel after implantation of the alloy into biological tissues. RESULTS AND CONCLUSION: Near-equiatomic nickel-titanium shape memory alloy has high nickel content. However, there has been controversy about the safety after long term implantation of the alloy in vivo. Alloy surface modification is an effective way to improve the biocompatibility of implants. The release of Ni ion can be decreased by surface modification of nickel-titanium shape memory alloy, and many related research works have been done by domestic and overseas scholars. However, studies about the metabolic process of Ni ion in vivo after implantation of nickel-titanium alloy are not comprehensive and systematic. Related Articles | Metrics

Select

Research progress and clinical application of mineral trioxide aggregate Zhu Lan-lan, Wang Xiu-mei 2012, 16 (25):  4692-4696.  doi: 10.3969/j.issn.1673-8225.2012.25.027 Abstract ( 240 )   PDF (507KB) ( 409 )   Save BACKGROUND: In recent years, a new-type dental material, mineral trioxide aggregate (MTA) emerges at home and aboard. It is widely used in clinical application because of its good physical and chemical properties and perfect biocompatibility. OBJECTIVE: To review the physical and chemical properties, biocompatibility and clinical application of MTA. METHODS: A computer-based research was performed on CNKI database and PubMed database (1993-01/2011-12) to search the related articles about MTA. The key words were “mineral trioxide aggregate, capping material, endodontic treatment” in English and Chinese. The related articles were selected. There are 68 articles after the initial survey. Then selecting 40 articles related MTA were concluded to review according to the conclusion criteria. RESULTS AND CONCLUSION: Application of MTA is a well adjuvant therapy for some special clinical pulposis. And it has attracted a wide spread attention by its good physical and chemical properties, closure property, biological activity and biological compatibility. The clinical application of MTA will be widely used along with the development of medicine and materials. Related Articles | Metrics

Select

Preparation of porous bio-ceramic beta-tricalcium phosphate scaffolds by freeze-drying technique Wang Xue-tao, Li Jing, Zuo Kai-hui, Zeng Yu-ping, Zhang Bao-wei 2012, 16 (25):  4697-4700.  doi: 10.3969/j.issn.1673-8225.2012.25.028 Abstract ( 275 )   PDF (524KB) ( 523 )   Save BACKGROUND: There are many factors which can influence the microstructures and properties of bio-ceramic scaffolds prepared by the freeze-drying process, such as the slurry solid loading, freezing rate, sintering conditions, and so on. OBJECTIVE: To prepare bio-ceramic β-tricalcium phosphate (β-TCP) scaffolds with different morphologies and porosities by adjusting the content of the slurries and analyze the influence factors. METHODS: The β-TCP scaffolds with different morphologies and porosities were prepared, and then bio-ceramic β-TCP scaffolds were obtained through freezing, drying and heating. Phase analysis, microstructure of the section and porosity of bio-ceramic β-TCP scaffolds were detected by X-ray diffraction, scanning electron microscopy and Archimedes drainage method, respectively. RESULTS AND CONCLUSION: The diffraction peak position of the scaffolds was identical with the standard β-TCP; the scaffolds without polyvinyl alcohol had the macroscopic lamellar pores and porous ceramic walls, and as the solid longing of slurry was increasing, the pore size and the porosity decreased; scaffolds containing polyvinyl alcohol: the lamellar pores gradually changed into the interconnected three-dimensional reticulate pores, and the porosity was improved because of the addition of polyvinyl alcohol. It is indicated the morphology and porosity of the bio-ceramic scaffolds prepared using the freeze-drying process can be controlled by adjusting the content of the slurries. Related Articles | Metrics

Select

10.3969/j.issn.1673-8225.2012.25.029 You Zuo-xiang, Yang Qin-zheng, Yue Zhan-guo, Ma Guang-hui, Wei Wei 2012, 16 (25):  4701-4705.  doi: 10.3969/j.issn.1673-8225.2012.25.029 Abstract ( 302 )   PDF (616KB) ( 735 )   Save BACKGROUND: The non-specific distribution of anticancer drugs in vivo may cause serious side effects and low bioavailability. Drug carriers with the active targeting abilities can solve these problems. OBJECTIVE: To review the recent studies on carrier preparation for anticancer drug delivery and summarize different types of active targeting strategies for drug carrier. METHODS: A computer-based online search was performed for articles published from 2004 to 2011 in Web of Knowledge database with the English key words that closely related to various active targeting strategies, such as “tumor, drug carrier, active targeting”. Totally 58 articles were retrieved, and finally 26 of them met the requirement. RESULTS AND CONCLUSION: Active targeting strategy is the first problem need to consider and the most important part in designing anticancer drug carriers. It contributes to the actual effect of drug delivery and bioavailability of drug. Drug carriers with various active targeting abilities can carry antitumor drugs to tumor sites and significantly reduced the non-specific distribution of drugs in vivo in order to decrease the side effects and enhanced bioavailability. Related Articles | Metrics

Select

Biological characteristics and clinical application of artificial hip joint weight-bearing surface materials Tang Kun 2012, 16 (25):  4706-4710.  doi: 10.3969/j.issn.1673-8225.2012.25.030 Abstract ( 271 )   PDF (684KB) ( 449 )   Save BACKGROUND: Artificial hip prosthesis materials play a decisive role in the success rates of hip replacement and the recovery rate for patients. OBJECTIVE: To evaluate the properties of hip joint weight-bearing surface materials with different combinations, as well as the biocompatibility of the materials and the host after implanted in vivo. METHODS: The key word of “total hip replacement, artificial hip joint, metal, ceramic, polyethylene, biocompatibility, clinical application” in Chinese and “total hip replacement, artificial hip, prosthetic materials, biocompatibility, clinical application” in English were used to retrieve the articles published from January 2001 to December 2011 in Wanfang and PubMed databases, respectively, by computer. Articles related to the biocompatibility of hip joint weight-bearing surface materials and their host were included. RESULTS AND CONCLUSION: Metal-ultra-high molecular weight polyethylene is commonly used in combination at present, which also measures other combinations as the gold standard. But its wear particles can induce peripheral tissue reactions, which lead to osteolysis and aseptic loosening of prosthesis. The combinations of metal-high cross linked polyethylene, metal-metal, ceramics-ceramics and ceramics-polyethylene all could reduce the attrition to a certain extent, but the metallic ion toxicity, ceramic fragility and high cost are still needed to solve. An ideal weight-bearing surface material should have good characteristics, such as biocompatibility, corrosion resistance, wear resistance, fatigue resistance and toughness. Currently, artificial hip joint bearing surface combinations have their advantages and disadvantages. Therefore, in order to achieve the best curative effect, clinicians should adopt an individualized therapeutic principle according different patients and consider patients with different illness and economic conditions to choose an appropriate prosthesis combination type. Related Articles | Metrics

Select

Improvement of the bond strength of porcelain-fused-to-metal restoration with different surface treatment technologies Na Tong, Cao Li-yan 2012, 16 (25):  4711-4714.  doi: 10.3969/j.issn.1673-8225.2012.25.031 Abstract ( 204 )   PDF (510KB) ( 394 )   Save BACKGROUND: Porcelain-fused-to-metal restoration is aesthetic, good organization compatibility and high strength, which is favored with the majority of patients. OBJECTIVE: To review the results of the improvement of the bond strength of porcelain-fused-to-metal with different surface treatment technologies in recently years. METHODS: A compute-based online retrieval of CNKI and OVID databases was performed for articles concerning the improvement of the bond strength of porcelain-fused-to-metal. The key words were “bond strength of porcelain, PFM, surface treatment technology, preoxidation, sol-gel, plasma-spray” in English, and “porcelain, PFM, surface treatment, preoxidation, sol-gel, plasma-spray” in Chinese. RESULTS AND CONCLUSION: The bond strength of porcelain-fused-to-metal is a key factor of successful application for restoration. Suitable use of surface treatment technology can improve the bond strength of porcelain-fused-to-metal, which could have a better repairing effect, such as: preoxidation, crytreat, sputtering Au intemadiate technology, plasma spraying technology, sol-gel technology, nano-medium material and vacuum deposition technology. These surface treatment technologies have improved the bond strength of porcelain-fused-to-metal at different degrees, but some technologies are still in the development and need to be further researched. Related Articles | Metrics

Select

Status and research progress of calcium phosphate bone cement Zheng Jiang-jiang, Bao Chong-yun 2012, 16 (25):  4715-4718.  doi: 10.3969/j.issn.1673-8225.2012.25.032 Abstract ( 236 )   PDF (507KB) ( 416 )   Save BACKGROUND: Calcium phosphate bone cement is a new kind of self-setting and injectable bone substitute material. Plenty of experiments have proved that there is a great progress in clinical areas of this material due to its good bioactivity, biocompatible and stable physical and chemical properties. OBJECTIVE: To summarize the status and research progress of calcium phosphate bone cement. METHODS: A computer-based online search of papers published from January 1999 to October 2011 related to new bone cement was performed in CNKI database, Science direct database and Pubmed database using the key words of “phosphates, silicates, bone cement, bone substitute” by screening titles and abstracts. The documents associated with calcium phosphate bone cement were selected, and those published recently or in authoritative journals were preferred in the same field. Totally 85 articles were obtained in initial retrieval, 13 of them were chosen to summarize based on inclusion criteria. RESULTS AND CONCLUSION: Calcium phosphate bone cement as a new kind of self-setting bio-material possesses better physical and chemical properties and biological properties compared with traditional bone cement materials. However, the researchers should pay more attention to investigate and improve the properties of this material in order to develop various types of calcium phosphate bone cement and afford novel ideas for repair of bone defects. Related Articles | Metrics

Select

Biological patch materials in pelvic functional reconstruction Wang Fang 2012, 16 (25):  4719-4726.  doi: 10.3969/j.issn.1673-8225.2012.25.033 Abstract ( 287 )   PDF (655KB) ( 410 )   Save BACKGROUND: Surgical treatment is the main method for treating pelvic organ prolapse resulting from structure defect, damage of pelvic floor, or pelvic floor dysfunction. With the development of technology in repair and functional reconstruction of pelvic floor defects, as an important pelvic fascia tissue substitute, biological patch materials are widely used in the pelvic floor reconstruction. OBJECTIVE: To explore the literature data trends in the study of biological patch materials in pelvic functional reconstruction. METHODS: A search of articles related to biological patch materials in pelvic functional reconstruction was performed in Wanfang database, with key words of “patch, pelvic functional reconstruction, pelvic organ bulging, biomaterial”, from 2007-01 to 2011-12. The distribution characteristics of research trends were described by using database analysis capabilities and Excel charting functions. The literatures were analyzed by annual number of hits, published year, literature number, subject categories, funds, as well as to compare the clinical effect of different patch materials in PhD thesis, master's degree papers, conference papers, and patented technology in Wanfang database. RESULTS AND CONCLUSION: A total of 30 research literatures were retrieved in Wanfang database from 2007 to 2011. The annual number of hits addressing literature research in patch materials showed a clear upward trend in recent 5 years, and the number of papers related to biological patch materials in pelvic functional reconstruction was in a steady state. Journal of Clinical Rehabilitative Tissue Engineering Research published more literatures than other journals. The pelvic organ prolapsed, polypropylene patch and biocompatibility are widely studied in the literatures addressing the application of biological patch materials in pelvic organ prolapse, and polypropylene patch material is the focus of the study, which highlights the biocompatibility of materials. The bibliometric analysis on biological patch materials in pelvic functional reconstruction in Wanfang database can provide valuable reference for Chinese medical workers in both basic research and clinical study of pelvic organ prolapse. Related Articles | Metrics

Select

Biocompatibility of different hemodialysis membrane materials used in renal failure patients: A comparative analysis Cao Ning 2012, 16 (25):  4727-4734.  doi: 10.3969/j.issn.1673-8225.2012.25.034 Abstract ( 270 )   PDF (695KB) ( 642 )   Save BACKGROUND: Hemodialysis is the main method for the treatment of acute and chronic renal failure; hemodialysis machine can purify the blood through diffusion, convection and filtration procedures by a semi-permeable membrane, and it can excrete the metabolic wastes and excess electrolyte in blood. Selection of the dialysis membrane is closely related with the effectiveness of hemodialysis. OBJECTIVE: To perform a multilevel analysis on the articles addressing hemodialysis membrane materials using the search and depth analysis capabilities of CNKI database. METHODS: The CNKI database was used to search articles related to hemodialysis membrane materials from January 2002 to December 2011 with the key words of “hemodialysis, dialysis membrane, biomaterials”. The publishing time, number, subject category, source journals, fund assistance, author distribution, research institution, key words, cited frequency and download frequency of the articles were analyzed. We expressed the articles through text and charts. RESULTS AND CONCLUSION: A total of 95 articles were included for the analysis. Judging from the number of the articles, there was 20 articles published in 2002; the number of the articles published in Chinese Journal of Blood Purification was up to 19. In view of the key words, hemodialysis membrane was mainly used for the treatment of uremia, and the analysis of the hemodialysis membrane was mainly focused on the biocompatibility of hemodialysis membrane materials, including the effect of dialysis membrane on the oxidative stress and phosphate removal of the patients with maintenance hemodialysis. The articles regarding hemodialysis membrane in CNKI database were analyzed by bibliometric method which could provide the research ideas and valuable data for the workers engaged in the basic research and clinical implementation of hemodialysis membrane materials. Related Articles | Metrics

Select

Application of titanium mesh materials in the repair of cranium damage Zhang Dong-qiang 2012, 16 (25):  4735-4744.  doi: 10.3969/j.issn.1673-8225.2012.25.035 Abstract ( 336 )   PDF (795KB) ( 622 )   Save BACKGROUND: Cranium damage is common in neurosurgery and plastic surgery, It cannot only relieve the intracranial hypertension, but also restore the shape of the skull, protect the brain tissue of the defect site, and restore the electroencephalogram and neuropsychological function, which has a great significance in improving the brain function. OBJECTIVE: To analyze the effect of titanium mesh materials on cranium damage. METHODS: The CNKI database was searched for the articles related to the application of titanium mesh materials in the repair of cranium damage from January 2002 to December 2011. The key words were “titanium mesh, cranium damage, biomaterial”. The articles were analyzed by the analysis functions of the database and the charting functions of Excel software, and the distribution characteristics were analyzed through texts and charts. The literature published number, subject category, source journals, foundation, research institutions and keyword, etc, were analyzed, as well as the doctoral dissertation, master degree thesis, important conference papers and patent information. The efficacy of titanium mesh and other different repairing materials was analyzed. RESULTS AND CONCLUSION: A total of 366 articles were screened out. In recent 10 years, the amount of the articles on the application of titanium mesh materials in the repair of cranium damage was increased. The key words of the articles were cranium damage, titanium mesh, and cranium repairing; the amount of the articles on the application of titanium mesh materials in the repair of cranium damage was the most, mainly in the analysis of contourable titanium mesh and digital formed titanium mesh. The articles related to the application of titanium mesh materials in the repair of cranium damage in CNKI database were analyzed through bibliometric method, which can provide valuable suggestions for the basic research and the clinical medical workers on the repair of cranium damage in neurosurgery and plastic surgery. Related Articles | Metrics

Select

Vacuum sealing drainage for skin avulsion of the limbs Xie Zhi-jin, Fang Yue, Wang Yan-chuan, Shi Lei, Yu Qiang, Liu Yong 2012, 16 (25):  4745-4750.  doi: 10.3969/j.issn.1673-8225.2012.25.036 Abstract ( 182 )   PDF (482KB) ( 335 )   Save BACKGROUND: Vacuum sealing drainage technique has been increasingly popular in clinical treatment of skin avulsion injuries. OBJECTIVE: To investigate and generalize technical issues and details of the vacuum sealing drainage method in the treatment of limb skin avulsion. METHODS: A total of 22 patients with limb skin avulsion were admitted and then treated with vacuum sealing drainage after skin replantation. Firstly, the main points and difficulties of the treatment process were retrospectively reviewed, and then the appropriate technical approach was proposed. RESULTS AND CONCLUSION: Although 21 eligible patients received effective treatment and the clinical results were quite satisfactory, there were six cases of failure to complete primary healing. All of these cases provide us a good clinical support for the summary of the related technical points. To conclude, skin replantation combined with vacuum sealing drainage technique is a good way to deal with limb skin avulsion, and a better grasp of technical issues and details of the approach helps improve the cure rate. Related Articles | Metrics