Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (25): 4566-4570.doi: 10.3969/j.issn.1673-8225.2012.25.002

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Effect of bone matrix gelatin/true bone ceramic on the osteogenic potential of bone marrow mesenchymal stem cells

Liu Xi-min1, Tian Zhi-feng1, Zhang Xiao-ling2, Wang Feng-gang1   

  1. 1Department of Anatomy, 2Department of Pathophysiology, Luohe Medical College, Luohe 462002, Henan Province, China
  • Received:2011-09-29 Revised:2011-11-03 Online:2012-06-17 Published:2013-11-04
  • About author:Liu Xi-min, Associate professor, Department of Anatomy, Luohe Medical College, Luohe 462002, Henan Province, China zhifeng_07@ sina.com

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Abstract:

BACKGROUND: Previous studies have found that simply bone matrix gelatin (BMG) material compound with bone marrow mesenchymal stem cells (BMSCs) has a good osteogenic potential for bone defects with better effects, but simply BMG cannot be used to repair bone defects in load-bearing area because of rapid degradation speed and bad hardness.
OBJECTIVE: To observe the changes in the osteogenic potential of BMSCs co-cultured with BMG/true bone ceramic (BMG/TBC).
METHODS: BMG and TBC were used to prepare recombinant bone that was co-cultured with BMSCs for 48 hours in vitro. Osteogenic potential of the recombinant bone was detected by Alizarin bordeaux staining.
RESULTS AND CONCLUSION: BMSCs in composited material could adhere, grow and proliferate well after co-cultured with BMG/TBC. There were no changes of the osteogenic potential and cell phenotype. It is indicated that BMG/TBC is easy to combined with BMSCs without affecting osteogenic potential, which shows a good biocompatibility.

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