Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (25): 4561-4565.doi: 10.3969/j.issn.1673-8225.2012.25.001

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Osteogenetic effect of Bio-Oss bone powder combined with bone marrow stromal stem cells

Li Xiang1, Huang Dai-ying2, Chen Song-ling2, Zhu Shuang-xi1   

  1. 1Department of Stomatology, Huangpu Branch, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China;
    2Department of Stomatology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China
  • Received:2011-12-06 Revised:2012-02-04 Online:2012-06-17 Published:2013-11-04
  • Contact: Chen Song-ling, Doctor, Professor, Doctoral supervisor, Department of Stomatology, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China chensongling@ hotmail.com
  • About author:Li Xiang☆, Doctor, Attending physician, Department of Stomatology, Huangpu Branch, First Affiliated Hospital of Sun Yat-sen University, Guangzhou 510080, Guangdong Province, China hpyylx@163.com

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Abstract:

BACKGROUND: The use of bone tissue engineering in maxillary sinus augmentation is a research hotspot in oral implantology at present.
OBJECTIVE: To investigate the osteogenic effect of bone tissue engineering on maxillary sinus floor augmentation with simultaneous dental implant.
METHODS: The bone marrow stromal stem cells were isolated from dogs and cultured in vitro. Then, the cells were cultured with Bio-Oss bone powder and induced to differentiate to osteoblasts. Twelve healthy adult dogs were subjected to bilateral maxillary sinus elevation with simultaneous dental implant. One side was implanted with the compound of bone marrow stromal stem cells and Bio-Oss bone power, and the other side was implanted with Bio-Oss bone power alone as controls.
RESULTS AND CONCLUSION: Gross observation showed that the implants were stable and not loose, as well as maxillary sinus mucosa was intact. New bone at the experimental side formed earlier and increased in bone mass, while new bone at the control side formed more slowly. X-ray examination showed that new bone at the experimental side was dense and combined tightly with implants. Biomechanical testing indicated that the pull-out force was increased with time, and there were significant differences between the experimental and control sides at weeks 12 and 24 (P < 0.05). Histomorphological analysis exhibited that new bone area was increased gradually, and there were significant differences between the experimental and control sides at weeks 12 and 24 (P < 0.05). These findings suggest that the tissue engineered bone can obtain a good effect on maxillary sinus augmentation with simultaneous dental implant.

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