Chinese Journal of Tissue Engineering Research ›› 2012, Vol. 16 ›› Issue (25): 4663-4666.doi: 10.3969/j.issn.1673-8225.2012.25.021

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Preparation and in vitro detection of osteogenic growth peptide chitosan-alginate microspheres

Ma Shu-qiang1, Peng Jian-qiang1, Li Zhong-tan1, Li Yu-hong1, Wu Wei-dong1, Lin Qi-sheng1, Wang Kun-zheng2   

  1. 1Department of Orthopedics, the Fourth People’s Hospital of Shenzhen, Shenzhen 518033, Guangdong Province, China;
    2Department of Orthopedics, the Second Hospital of Xi’an Jiaotong University, Xi’an 710004, Shaanxi Province, China
  • Received:2011-11-11 Revised:2011-12-20 Online:2012-06-17 Published:2013-11-04
  • About author:Ma Shu-qiang☆, Doctor, Associate chief physician, Department of Orthopedics, the Fourth People’s Hospital of Shenzhen, Shenzhen 518033, Guangdong Province, China mashuqiang369@ 163.com

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Abstract:

BACKGROUND: In vitro injection of osteogenic growth peptide (OGP) can regulate the peripheral blood and proliferation of bone marrow cells to increase bone formation and accelerate fracture healing, but it can not be worldly used for its instability and inconvenience of injection.
OBJECTIVE: To prepare the OGP chitosan-alginate microsphere by emulsion crosslinking, and to detect its particle size, drug loading, in vitro release, physical and chemical properties.
METHODS: The OGP loaded chitosan-alginic acid microsphere was prepared using an emulsion cross-linking method with glutaral as the cross-linking agent. Microscopy and scanning electron microscopy were used to evaluate the morphological properties and particle size distribution. The drug loading, entrapment efficiency and in vitro drug release kinetics were detected by enzyme-linked immunosorbent assay.
RESULTS AND CONCLUSION: The OGP loaded chitosan-alginic acid microspheres were spherical in shape, and showed a asperity surface with many micropores and a higher encapsulation efficiency (> 72%). From the in vitro release experiment, the OGP was controlled to release from the chitosan microsphere over 49 days and the release property of microspheres in vitro was well and the overall release rate was > 85%. It is indicated that the OGP loaded chitosan-alginic acid microsphere that is prepared by emulsion cross-linking method shows a good controlled release property.

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