Abstract:

BACKGROUND: Acellular dermal matrix has a good biocompatibility and the inductive effect on tissues or cells.
OBJECTIVE: To evaluate the biocompatibility of acellular dermal matrix with human adipose-derived stem cells (ADSCs).
METHODS: Human ADSCs were isolated from the subcutaneous adipose tissue of healthy adult after liposuction, and primary culture and subculture of human ADSCs were conducted. Human ADSCs of passage 3 were co-cultured with the acellular dermal matrix in vitro for 3-7 days. Cell growth, proliferation and adhesion on the scaffolds were observed under inverted phase contrast microscope and scanning electron microscope, and the adhesion rate was analyzed. XTT-colorimetry was used to observe the cell growth and proliferation.
RESULTS AND CONCLUSION: Human ADSCs distributed evenly on the acellular dermal matrix scaffold. The cells expanded and adhered on the scaffold within 24 hours. The cells showed extensional deformation at 2-3 days, and the cell shape was spindle in main and mesh arranged. Cells on the scaffold were gradually increased as time went on. The average adherens rate was 95.03% of ADSCs co-cultured with acellular dermal matrix scaffold, and the cell growth and proliferation were normal. It is indicated that ADSCs can well attached to acellular dermal matrix scafold and normally grow and proliferate on the scaffolds. Acellular dermal matrix scaffold has a good biocompatibility with ADSCs.