Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7525-7528.doi: 10.3969/j.issn.1673-8225.2011.40.026

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Influence of stem cells transplantation on cochlear structure and hearing function of guinea pigs

Chen Guan-gui1, Xu Hui-qing2   

  1. 1Department of Otorhinolaryngology, the Second Affiliated Hospital of Guangzhou Medical College, Guangzhou  510260, Guangdong Province, China
    2Department of Otorhinolaryngology-Head and Neck Surgery, the Third Affiliated Hospital of Sun Yat-sen University, Guangzhou  510260, Guangdong Province, China
  • Received:2011-04-04 Revised:2011-05-23 Online:2011-10-01 Published:2011-10-01
  • About author:Chen Guan-gui☆, Doctor, Attending physician, Department of Otorhinolaryngology, the Second Affiliated Hospital of Guangzhou Medical College, Guangzhou 510260, Guangdong Province, China entcgg@yahoo.com.cn

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Abstract:

BACKGROUND: It has been proved that the influence to cochlear structure and hearing function is mild after gene therapy by virus or non-virus vector via cochlear micro-windowing.
OBJECTIVE: To explore the changes in function and structure of recipients inner ear of the normal guinea pigs by transplantation of bone marrow mesenchymal stem cells (BMMSCs) via cochlear micro-windowing.
METHODS: The normal guinea pigs were divided into three groups randomly. Group 1 served as a negative control without any intervention, group 2 were performed micro-windowing without any injection, and group 3 were injected BMMSCs labeled by DAPI. Preoperative and postoperative ABR audibility thresholds were compared between and within groups. Cochlear structures were evaluated by microscopic examination of paraffin sections cut after hematoxylin-eosin staining. The distribution and survival of transplanted cells was evaluated by fluorescence microscope.
RESULTS AND CONCLUSION: Auditory brainstem response thresholds elevated significantly 7 days after operation in group 2 and group 3 as compared with group 1, but recovered within 28 days. No distinct structural alternations were found in cochlear paraffin sections. Most transplanted cells were found in the scala tympani and scala vestibule. The influence to hearing function and cochlear structure was mild after transplantation of BMMSCs via cochlear micro-windowing. The grafted cells could survive in the cochleas and had the potential to migrate.

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