Chinese Journal of Tissue Engineering Research ›› 2011, Vol. 15 ›› Issue (40): 7520-7524.doi: 10.3969/j.issn.1673-8225.2011.40.025

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Tail vein injection of bone marrow stromal cells versus intraperitoneal injection of granulocyte colony-stimulating factor in the treatment of cerebral infarction in rats

Gu Ping1, 2, Zhang Zhong-xia1, Zhou Mei-gang2, Wang Yan-yong1, 2, Dong Ci2, Cui Dong-sheng1, Geng Yuan1, Wang Ming-wei1, 2   

  1. 1Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province, Shijiazhuang  050031, Hebei Province, China
    2Department of Neurology, First Hospital of Hebei Medical University, Shijiazhuang  050031, Hebei Province, China
  • Received:2011-03-11 Revised:2011-04-19 Online:2011-10-01 Published:2011-10-01
  • Contact: Wang Ming-wei, Doctor, Chief physician, Professor, Doctoral supervisor, Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province, Shijiazhuang 050031, Hebei Province, China; Department of Neurology, First Hospital of Hebei Medical University, Shijiazhuang 050031, Hebei Province, China
  • About author:Gu Ping☆, Doctor, Chief physician, Professor, Master’s supervisor, Brain Aging and Cognitive Neuroscience Laboratory of Hebei Province, Shijiazhuang 050031, Hebei Province, China; Department of Neurology, First Hospital of Hebei Medical University, Shijiazhuang 050031, Hebei Province, China gpwh2000@yahoo.com.cn
  • Supported by:

    the Key Basic Research Project of Hebei Provincial Applied Basic Research Planning, No. 08966102D*

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Abstract:

BACKGROUND: Bone marrow stem cells (BMSCs) possess the potency of transverse differentiation and the character of nerve formation. There are a few researches at intravenous BMSCs and intraperitoneal injection of granulocyte colony stimulating factor (G-CSF) to mobilize stem cells on treatment of cerebral artery occlusion model (MCAO) in rats.
OBJECTIVE: To compare the effects of tail vein injection of BMSCs versus intraperitoneal injection of G-CSF in the treatment of cerebral infarction in rats.
METHODS: In this experiment, modified Zea-longa suture method was applied on the SD rats to block the middle cerebral artery, thus to establish the model of cerebral infarction. BMSCs were injected via the tail vein and G-CSF was injected intraperitoneally at 24 hours after modeling.
RESULTS AND CONCLUSION: Cognitive and motor function could be improved by G-CSF of intraperitoneal injection and BMSCs of tail vein injection in rats. The improvement of cognitive and motor function of MCAO rats of G-CSF was significantly higher than that of BMSCs by the tail vein injection. At 7 and 14 days after transplantation, the infarct area in rats undergoing BMSCs transplantation was bigger than that of rats undergoing G-CSF (P < 0.05), and the number of BrdU positive cells in the G-CSF group was higher than that in the BMSCs group (P < 0.05). G-CSF can promote BMSCs to migrate to the corpus callosum. The results suggest that the method of G-CSF by mobilizing BMSCs may be better than BMSCs transplantation alone for treatment of cerebral ischemia.

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