Abstract:

BACKGROUND: In the past, only vascular tissues on fat surface were removed during the primary culture of adipose tissue-derived stem cells (ADSCs) reported in most literatures. However, blood vessels within the fat tissues, especially the lymph nodes around the blood vessels removed little.
OBJECTIVE: To investigate the feasibility of raising purity of cultured ADSCs in the laboratory through purifying adipose tissues.
METHODS: Adipose tissues derived from rat inguinal groove were divided into two parts: one for purifying, rejecting superficial blood vessel, skins and muscular tissues, rejecting blood vessel inside and surrounding elliptic nodal tissues; the other one only for rejecting superficial blood vessels and possible adherent skins and muscular tissues outside. Primary culture of ADSCs was performed.
RESULTS AND CONCLUSION: Elliptic nodal tissues stained by hematoxylin-eosin were proved to be lymphatic tissues under microscope. The cells cultured from purified adipose tissues were uniformity. Cell photos, immunofluorescence and flow cytometry results can prove that the cell’s purity from the purified adipose tissues was higher than that from the unpurified adipose tissues. The purity of primary cultured ADSCs can be improved by purifying adipose tissues in the laboratory.