Mechanism of Qutan Zhuyu Decoction for osteonecrosis of the femoral head based on network pharmacology

doi:10.3969/j.issn.2095-4344.2875

Abstract

Abstract:

BACKGROUND: Traditional Chinese medicine believes that the early bone erosions mostly belong to phlegm and blood stasis, so early treatment of osteonecrosis of the femoral head (ONFH) is often similar to the treatment of phlegm and blood stasis. Based on network pharmacology we can build a “component-target- pathway-disease” network of drugs to explore the relationship between drugs and diseases, thereby clarifying the mechanism of drugs

OBJECTIVE: To construct the component-target-pathway-disease network of Qutan Zhuyu Decoction and to explore the key targets and mechanism of Qutan Zhuyu Decoction in the treatment of ONFH.

METHODS: Taohong Siwu Decoction and Erchen Decoction are effective prescriptions for the treatment of early ONFH. The representative drugs include Pinellia tangerine peel, medlar, peach kernel and safflower, named as Qutan Zhuyu Decoction. The main active components and targets of Qutan Zhuyu Decoction was searched and screened in the Chinese Medicine Computing System Pharmacology Analysis Platform (TCMSP), and a search for ONFH targets in the Human Gene Database (GeneCards) and the Human Mendelian Genetic Database (OMIM) was performed. The common targets of Qutan Zhuyu Decoction and ONFH were got by Venn diagrams. Cytoscape3.6.1 was used to construct a Chinese medicine-ingredient-disease-target regulation network, then establish a protein-protein interaction network through the STRING data platform, and finally use the Bioconductor platform and R language for gene ontology enrichment analysis and gene interaction (KEGG) pathway analysis.

RESULTS AND CONCLUSION: The main components of Qutan Zhuyu Decoction for the treatment of ONFH were 78 active components such as sitosterol and naringenin. The targets for therapeutic effects might include 61 key targets such as NCOA2 and PTGS1. The biological processes affecting the treatment of ONFH mainly included proximal promoters, sequence-specific DNA binding, and regulation of DNA-binding transcriptional activity. Key pathways for therapeutic use included interleukin-17 signaling pathway and apoptosis. Overall, the therapeutic effect of Qutan Zhuyu Decoction on ONFH reflects the characteristics of multi-component-multi-target-multi-channel of traditional Chinese medicine, suggesting that its mechanism may be achieved by multi-components, multi-targets and multi-channels.

Key words: Qutan Zhuyu Decoction, osteonecrosis of the femoral heads, mechanism, network pharmacology

CLC Number:

Fan Yinuo, Chen Minghai, Li Weifeng, Chen Lixin, Wei Qiushi, He Wei, Chen Zhenqiu. Mechanism of Qutan Zhuyu Decoction for osteonecrosis of the femoral head based on network pharmacology[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(35): 5638-5643.

Figures/Tables 8

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