Common mechanism of Chinese herbs for promoting blood circulation and removing blood stasis in the treatment of osteonecrosis of the femoral head: an analysis based on network pharmacology

doi:10.3969/j.issn.2095-4344.3167

Abstract

Abstract: BACKGROUND: Chinese herbs for promoting blood circulation and removing blood stasis are the most commonly used Chinese medicines for the treatment of osteonecrosis of the femoral head (ONFH), while Chuanxiong, Achyranthes bidentata, Salvia miltiorrhiza, rhizoma drynariae, and safflower are the most commonly used herbs for treating ONFH.
OBJECTIVE: To explore the common mechanism of five Chinese herbs for promoting blood circulation and removing blood stasis in the treatment of ONFH
METHODS: The main active ingredients of five herbs were obtained by searching the database of TCM system pharmacology analysis platform (TCMSP), and the gene targets of five herbs were speculated. Then the potential gene targets of all five traditional Chinese herbs could be obtained by taking the intersection function through the Excel table. Relevant targets of ONFH disease were obtained through GeneCards and OMIM databases. Chinese herbs for promoting blood circulation and removing blood stasis-therapeutic ingredients-targets-ONFH network was constructed, followed by gene ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis.
RESULTS AND CONCLUSION: A total of 132 active ingredients of five herbs were screened out through the TCMSP database search, with 2 593 potential target genes correspondingly. There were 31 common potential targets of these five herbs. A search of OMIM and GeneCards databases yielded 705 ONFH disease-related targets, and 8 intersected genes (PPARG, NOS3, F2, NCOA2, NOS2, GSK3B, ESR1, NR3C1) were obtained. GO biological analysis showed that the main biological process of five Chinese herbs in ONFH was related to negative regulation of platelet activation, nitric oxide biosynthesis, and positive regulation of vasodilation. The KEGG pathway analysis showed that the main pathway of five Chinese herbs in ONFH was related to Thyroid hormone signaling pathway, hypoxia-inducible factor-1 signaling pathway, prolactin signaling pathway, and estrogen signaling pathway. The possible main mechanisms of five Chinese herbs in the treatment of ONFH may be: the effective ingredients in herbs such as quercetin, berberine, coptisine, miltirone, by acting on ESR1, NOS3, NOS2, PPARG and other targets, participate in the regulation of thyroid hormone signaling pathway, hypoxia-inducible factor-1 signaling pathway, prolactin signaling pathway, and estrogen signaling pathway, and by regulating the activity of osteoblast and osteoclast, they can improve the hypercoagulability of blood and vascular permeability and promote angiogenesis, further promoting the repair of the necrotic femoral head to achieve the therapeutic effect. However, this study only predicted the mechanism of action, and the specific mechanism of action still needs to be verified by follow-up experiments.

Key words: bone, promoting blood circulation and removing blood stasis, Chinese herbs, osteonecrosis of the femoral head, osteoclast, vasodilatation, pathway, network pharmacology

CLC Number:

Xin Pengfei, Ke Mengnan, Zhang Haitao, Urishana, Li Ziqi, Zhuang Zhikun, Wei Qiushi, He Wei. Common mechanism of Chinese herbs for promoting blood circulation and removing blood stasis in the treatment of osteonecrosis of the femoral head: an analysis based on network pharmacology[J]. Chinese Journal of Tissue Engineering Research, 2021, 25(17): 2727-2733.

Figures/Tables 8

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