Preparing an adult mouse model of functional dyspepsia

doi:10.3969/j.issn.2095-4344.2805

Abstract

Abstract:

BACKGROUND: A suitable rat model of functional dyspepsia is a reliable animal model for subsequent clinical treatment and drug research.

OBJECTIVE: To prepare a model of functional dyspepsia in adult mice using a combined multi-factor method.

METHODS: Sixty Kunming mice were randomly divided into a blank control group and a model group, with 30 mice in each group. The mice in the blank control group were orally administered 0.2 mL of a 2% sucrose solution for 8 continuous days. Based on the classic iodoacetamide modeling method combined with daily feeding, the model group was subjected to force swimming in a water tank for 20 minutes, and 1 hour later, the mice were orally administered 5.7 g/kg L-arginine solution for 5 days, followed by oral administration of cold rhubarb decoction 2.9g/gk for another 3 days. The behavioral and pathological changes under light microscope were observed to determine the modeling effect. The study protocol was approved by the Animal Ethic Committee of Chengde Medical College, No. CDMULAC-20191113-011.

RESULTS AND CONCLUSION: Compared with the control group, the model group showed a reduction in hair color, arch back and body mass, and the intestinal propulsion rate and gastric emptying rate both had a downtrend. Intestinal tissue sections showed mild damage under the light microscope. Therefore, the combined multi-factor modeling method used in in the adult mice can successfully prepare the functional dyspepsia model. Moreover, the animal model prepared by this method has no obvious organic lesions, such as necrosis and erosion, indicating that this is a feasible method to prepare the adult mouse model of functional dyspepsia.

Key words: dyspepsia, gastrointestinal, mouse, experiment, animal, model

CLC Number:

Yu Jing, Pang Jiayu, Jia Ziye, Wu Xiaoguang. Preparing an adult mouse model of functional dyspepsia[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(32): 5158-5161.

Figures/Tables 5

References

[1] 李建锋,谢胜,陈广文,等.碘乙酰胺在消化系统疾病动物模型研究中的应用概况[J].中国实验动物学报,2018,26(4):533-539.

[2] 吕林,唐旭东,王凤云,等.胃动力障碍型功能性动物模型的建立[J].中国中西医结合杂志,2017,37(8):944-949.

[3] 刘成全,邓青,谭志超,等.功能性消化不良脾虚证大鼠模型的建立及评价[J].中国实验动物学报,2017,25(3):311-315.

[4] WEI W, LI X, HAO J, et al.Proteomic analysis of functional dyspepsia in stressed rats treated with traditional Chinese medicine "Wei Kangning".J Gastroenterol Hepatol. 2011;26(9): 1425-1433.

[5] LIANG Q, YAN Y, MAO L,et al.Evaluation of a modified rat model for functional dyspepsia.Saudi J Gastroenterol.2018;24(4): 228-235.

[6] 刘红静.益胃散对功能性消化不良模型小鼠血浆胃动素水平的影响[D].哈尔滨:黑龙江中医药大学,2008.

[7] 吴洋,马学琴,王洋,等.胃元宁对功能性消化不良小鼠胃肠运动及胃肠激素分泌的影响[J].中国实验方剂学杂志,2014,20(24):185-189.

[8] YE Y, WANG XR, ZHENG Y, et al. Choosing an Animal Model for the Study of Functional Dyspepsia.Can J Gastroenterol Hepatol. 2018;2018:1531958.

[9] 林雪莲,唐旭东,王凤云,等.肝郁脾虚与胃肠运动障碍关系的研究[J].中国中西医结合消化杂志,2016,24(10):805-808.

[10] 韩荣燕,吕康,刘树丽,等.功能性消化不良的中西医诊治进展[J].长春中医药大学学报,2018,34(4):809-812.

[11] 刘俊宏,汪龙德,毛兰芳,等.功能性消化不良中医药研究进展[J].甘肃中医药大学学报,2017,34(6):91-94.

[12] 奚锦要,朱永钦,朱永苹,等.功能性消化不良中医药临床研究进展[J].辽宁中医药大学学报,2017,19(2):83-86.

[13] 戴宁.功能性消化不良的中医辨治经验总结[A].中国残疾人康复协会中医康复委员会.中国残疾人康复协会中医康复委员会第十三届学术年会论文集[C].中国残疾人康复协会中医康复委员会:中国残疾人康复协会中医康复委员会,2018:8.

[14] LUO J, WANG T, LIANG S, et al. Experimental gastritis leads toanxiety- and depression-like behaviors in female but not male rats, Behav Brain Funct.2013;9:46.

[15] 曹京梅,官莉.心理干预改善功能性消化不良患者焦虑、抑郁状态的效果观察[J].心理月刊,2019,14(13):62.

[16] 吕林,唐旭东,王凤云,等.胃动力障碍型功能性消化不良动物模型的建立[J].中国中西医结合杂志,2017,37(8):944-949.

[17] LIU LS, WINSTON JH, SHENOY MM, et al. A rat model of chronic gastric sensorimotor dysfunction resulting from transient neonatal gastric irritation.Gastroenterology.2008;134(7):2070-2079.

[18] FIORUCCI S, DISTRUTTI E, QUINTIERI A, et al. L-Arginine/Nitric Oxide Pathway Modulates Gastric Motility and Gallbladder Emptying Induced by Erythromycin and Liquid Meal in Humans. Dig Dis Sci. 1995;40(6):1365-1371

[19] 杜怡雯,冯江毅,胡黎文,等.大黄的药理活性研究及临床应用[J].中医临床研究,2018,10(25):24-27.

[20] 胡洁,彭买姣,罗怀浩,等.番泻叶造模对小鼠肠道微生物及酶活性的影响[J].中国微生态学杂志，2018,30(2):155-157

[21] 刘艳阳.功能性消化不良脾虚证动物模型制作及评价方法研究[D].北京:北京中医药大学,2015.

[22] 朱曼,康荻,张慧,等.脾虚复合肺纤维化影响大鼠肺功能的初步研究[J].北京中医药大学学报,2017,40(2):130-134.

[23] 陈艳琰,曹玉洁,唐于平,等.甘草调和大黄“泻下攻积”作用的量-毒-效关系研究[J].中国中药杂志,2019,44(10):2181-2189.

[24] 魏连波,刘玲玉.大黄的毒副作用及在肾病中的合理应用[J].中华肾病研究电子杂志,2018,7(1):13-16.