Chinese Journal of Tissue Engineering Research ›› 2020, Vol. 24 ›› Issue (19): 3010-3016.doi: 10.3969/j.issn.2095-4344.2070
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Zhu Haizhou1, 2, Zhao Zhankui1, Wang Xinzhe1, Liu Deqian1, Yu Honglian3
Received:
2019-07-08
Revised:
2019-07-09
Accepted:
2019-10-09
Online:
2020-07-08
Published:
2020-04-08
Contact:
Zhao Zhankui, MD, PhD, Associate professor, Department of Urology, Affiliated Hospital of Jining Medical University, Jining 272029, Shandong Province, China
About author:
Zhu Haizhou, Master, Professor, Department of Urology, Affiliated Hospital of Jining Medical University, Jining 272029, Shandong Province, China; Department of Surgery Pandect, Clinical Medical School, Jining Medical University, Jining 272067, Shandong Province, China
Supported by:
CLC Number:
Zhu Haizhou, Zhao Zhankui, Wang Xinzhe, Liu Deqian, Yu Honglian. Urine-derived stem cells differentiate into urothelial cells and smooth muscle cells in vitro[J]. Chinese Journal of Tissue Engineering Research, 2020, 24(19): 3010-3016.
2.4 尿源性干细胞向尿路上皮细胞诱导分化结果 尿源性干细胞经尿路上皮诱导专用培养基诱导分化14 d后,倒置显微镜下可见细胞形态变成“鹅卵石”样,见图4A,B。免疫细胞化学染色可见诱导组Cytokeratin 20表达阳性(++)和Uroplakin Ⅲ蛋白表达强阳性(+++),见图4C,D。荧光定量PCR结果显示诱导组尿路上皮细胞特异性基因Cytokeratin 7、Cytokeratin 20和Uroplakin Ⅲ表达显著高于对照组(P < 0.001),见图5A。Western blot结果显示诱导组Cytokeratin 7、Cytokeratin 20和Uroplakin Ⅲ蛋白表达水平显著高于对照组,见图5B。以上证实尿源性干细胞经体外诱导后可以表达尿路上皮细胞特异性表型蛋白,初步向尿路上皮细胞诱导分化成功。 "
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