Osteopontin promotes chondrocyte proliferation in osteoarthritis

doi:10.3969/j.issn.2095-4344.2017.24.001

Abstract

Abstract:

BACKGROUND: Osteopontin, a kind of extracellular matrix glycoprotein, has been found to participate in synthesis and catabolism of osteoarthritic chondrocyte extracellular matrix. However, the effect of osteopontin on the proliferation ability of osteoarthritic chondrocytes is little reported.

OBJECTIVE: To investigate the effect of osteopontin on the chondrocyte proliferation in human knee osteoarthritis.

METHODS: Cartilage samples were obtained from the patients with knee osteoarthritis undergoing knee arthroplasty at the Xiangya Hospital from January 2012 to June 2012. The chondrocytes were isolated and cultured, and then divided into four groups: blank control, osteopontin, Con-shRNA and osteopontin-shRNA groups. The cell proliferation was detected by MTT and BrdU assays.
RESULTS AND CONCLUSION: After transfection of osteopontin-shRNA lentivirus, the infection rate was up to 80%. Compared with the blank control group, osteopontin group showed a significant increase in the absorbance value of osteoarthritic chondrocytes, while after osteopontin-shRNA lentivirus transfection, the absorbance value was significantly decreased (both P < 0.05). Additionally, after osteopontin-shRNA transfection, the expression level of osteopontin was significantly downregulated (P < 0.05). To conclude, osteopontin can promote the proliferation of osteoarthritic chondrocytes, which is considered as a new treatment target for osteoarthritis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteopontin, Osteoarthritis, Chondrocytes, Cell Proliferation, Tissue Engineering

CLC Number:

R318

Jiang Wei, Lin Bo-wen, Zhang Xuan, Wang Hua, Gao Shu-guang, Zhang Fang-jie. Osteopontin promotes chondrocyte proliferation in osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(24): 3773-3777.

Figures/Tables 1

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