Hydrogen sulfide promotes the osteogenesis of bone marrow mesenchymal cells through hypoxia-inducible factor-1 alpha under tensile stress

doi:10.3969/j.issn.2095-4344.2017.21.001

Abstract

Abstract:

BACKGROUND: Hydrogen sulfide signaling has been proved to promote distraction osteogenesis; however, the underlying mechanism remains unclear.

OBJECTIVE: To evaluate the relationship between hydrogen sulfide and hypoxia-inducible factor-1α (HIF-1α) during the osteogenesis of rat bone marrow mesenchymal cells under tensile stress.
METHODS: 2 000 μ strain was loaded on the in vitro cultured rat bone marrow mesenchymal cells by a four-point bending apparatus, and hydrogen sulfide donor or HIF-1α inhibitor was adopted in the tensile unit. Subsequently, the levels of osteogenic markers were detected.
RESULTS AND CONCLUSION: Exogenous hydrogen sulfide signaling could promote the osteogenesis of rat bone marrow mesenchymal cells under tensile stress. However, this promotion was obviously eliminated when the endogenous HIF-1α expression was inhibited. These results show that hydrogen sulfide signaling system promotes the osteogenesis of rat bone marrow mesenchymal cells under tensile stress probably through HIF-1α.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Tissue Engineering, Hydrogen Sulfide, Hypoxia-Inducible Factor 1, alpha Subunit

CLC Number:

R318

Jiang Xiao-wen, Zhang Yi, Fan Xiao-sheng, Chen Yan-ze. Hydrogen sulfide promotes the osteogenesis of bone marrow mesenchymal cells through hypoxia-inducible factor-1 alpha under tensile stress[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(21): 3281-3286.

Figures/Tables 1

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