Influence of different intensities of extracorporeal shock waves on the osteogenesis ability of alveolar osteoblasts

doi:10.3969/j.issn.2095-4344.2017.16.009

Abstract

Abstract:

BACKGROUND: Oral inflammatory diseases usually cause alveolar bone loss and odontoseisis, and further impact dental occlusion. Extracorporeal shock wave therapy (ESWT) is a promising method for the repair of alveolar bone and improving osteogenic activity of alveolar osteoblasts, but its therapeutic efficacy and related mechanisms remain unclear.

OBJECTIVE: To compare the effect of different intensities of ESWT on the proliferation and osteogenesis abilities of rat alveolar osteoblasts.

METHODS: The rat alveolar osteoblasts were obtained and cultured in vitro, and further identified by alkaline phosphatase staining. 0.18, 0.36, and 0.50 mJ/mm² ESWT was used to stimulate the rat alveolar osteoblasts, 100 pulses, respectively.

RESULTS AND CONCLUSION: The levels of alkaline phosphatase and bone morphogenetic protein 2 were significantly increased in the 0.36 and 0.18 mJ/mm² ESWT groups, especially in the 0.36 mJ/mm² ESWT group (P < 0.05). 0.50 mJ/mm² ESWT significantly decreased the proliferation ability of rat alveolar osteoblasts and downregulated the levels of alkaline phosphatase and bone morphogenetic protein 2 (P < 0.05). To conclude, ESWT (< 0.36 mJ/mm²) can improve the osteogenesis ability of rat alveolar osteoblasts with the intensity increasing, which provides a theoretical basis for the clinical use of ESWT in the alveolar bone repair.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: High-Energy Shock Waves, Cell proliferation, Tissue Engineering

CLC Number:

R318

Guo Yuan, Zhang Chi, Liu Song, Wang Zhao, Pan Xing-fei, Wang Le. Influence of different intensities of extracorporeal shock waves on the osteogenesis ability of alveolar osteoblasts[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(16): 2509-2514.

Figures/Tables 1

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