Correlation between resistin level and severity of knee osteoarthritis

doi:10.3969/j.issn.2095-4344.2017.16.001

Abstract

Abstract:

BACKGROUND: Resistin has been shown to play an important role in the pathogenesis and progression of osteoarthritis.
OBJECTIVE: To investigate the correlation between resistin level in serum and synovial fluid and the severity of knee osteoarthritis.
METHODS: Seventy-four patients with knee osteoarthritis and 79 healthy individuals were recruited. The Noyes score method was used to assess articular cartilage damage arthroscopically. The severity of knee osteoarthritis was evaluated according to the Western Ontario McMaster University Osteoarthritis (WOMAC) score. The radiographic severity of knee osteoarthritis was assessed by the Kellgren-Lawrence (K-L) grading system. The levels of resistin and cross linked C-telopeptide of type II collagen (CTX-II) in serum and synovial fluid were determined by ELISA.
RESULTS AND CONCLUSION: The resistin level in the synovial fluid was positively correlated with the radiographic severity of knee osteoarthritis, WOMAC pain, WOMAC function, and WOMAC total scores, but showed no correlation with the WOMAC stiffness score. There were no significant correlations between the serum level of resistin and WOMAC total scores, subscale or K-L Grading system in the the osteoarthritis patients. Multiple regression analysis showed that the correlation between the synovial fluid level of resistin and WOMAC scores was of significance after removal of other confounding factors, and the resistin level in the synovial fluid was positively correlated with Noyes scores and CTX-II level. These results suggest that the resistin level in the synovial fluid can serve as a potential biomarker reflecting the severity of knee osteoarthritis and cartilage degenerative degree in patients with osteoarthritis.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Osteoarthritis, Resistin, Synovial Fluid, Biological Markers, Tissue Engineering

CLC Number:

R318

Song Yong-zhou, Guan Jian, Li Ming, Ma Wei, Tong Jiu-hui, Su Rui-hong. Correlation between resistin level and severity of knee osteoarthritis[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(16): 2461-2466.

Figures/Tables 3

References

[1] Goldring MB. The role of the chondrocyte in osteoarthritis. Arthritis Rheum.2000;43(9):1916-1926.

[2] Kraus VB,Nevitt M,Sandell LJ.Summary of the OA Biomarkers Workshop 2009-biochemical biomarkers: biology, validation, and clinical studies. Osteoarthritis Cartilage. 2010; 18(6): 742-745.

[3] Kraus VB, Burnett B, Coindreau J, et al.Application of biomarkers in the development of drugs intended for the treatment of osteoarthritis.Osteoarthritis Cartilage.2011;19(5): 515-542.

[4] Kang EH, Lee YJ, Kim TK,et al.Adiponectin is a potential catabolic mediator in osteoarthritis cartilage.Arthritis Res Ther.2010;12(6):R231

[5] McNulty AL, Miller MR, O'Connor SK,et al.The effects of adipokines On cartilage and meniscus catabolism. Connect Tissue Res.2011;52(6):523-533.

[6] Steppan CM, Brown EJ,Wright CM,et al.A family of tissue-specific resistin-like molecules. Proc Natl Acad Scit USA.2001;98(2):502-506.

[7] Nogueiras R, Gallego R, Gualillo O，et al. Resistin is expressed in different rat tissues and is regulated in a tissue-and gender-specific manner. FEBS Lett 2003;548 (1-3):21-27.

[8] Savage DB, Sewter CP, Klenk ES,et al.Resistin/Fizz3 expression in relation to obesity and peroxisome proliferator-activated receptor-gamma action in humans. Diabetes.2001;50(10):2199-2202.

[9] Bokarewa M,Nagaev I,Dahlberg L,et al.Resistin, an adipokine with potent prointlammtory properties.J Immunol.2005; 174(9): 5789-5795.

[10] Zhang Z,Xing X,Hensley G,et al.Resistin induces expression of prointlammatory cytokines and chemokines in human articular chondrocytes via transcription and messenger RNA stabilization. Arthritis Rheum.2010;62(7): 1993-2003.

[11] Lee JH, Ort T, Ma K,et al. Resistin is elevated following traumatic joint injury and causes matrix degradation and release of inflammatory cytokines from articular cartilage in vitro.Osteoarthritis Cartilage.2009;17(5):613-620.

[12] Filkova M, Liskova M, Hulejova H,et al.Increased serum adiponectin levels in female patients with erosive compared with non-erosive osteoarthritis.Ann Rheum Dis.2009;68(2): 295-296.

[13] Perruccio AV,Mahomed NN, ChandranV,et al.Plasma adipokine levels and their association with overall burden of painful joints among individuals with hip and knee osteoarthritis. J Rheumatol.2014;41(2): 334-337.

[14] Bellamy N,Buchanan WW,Goldsmith CH,et al. Validation study of WOMAC: a health status instrument for measuring clinically important patient relevant outcomes to antirheumatic drug therapy in patients with osteoarthritis of the hip or knee. J Rheumatol.1988;15(12):1833-1840.

[15] Leach RE, Gregg T, Siber FJ. Weight-bearing radiography in osteoarthritis of the knee.Radiology.1970;97(2):265-268.

[16] Kellgren JH,Lawrence JS.Radiological assessment of osteoarthrosis.Ann Rheum Dis.1957;16(4):494-502.

[17] Engstrom G,Gerhardsson de Verdier M,Rollof J,et al. C-reactive protein, metabolic syndrome and incidence of severe hip and knee osteoarthritis. A population-based cohort study.Osteoarthritis Cartilage.2009;17(2):168-173.

[18] Juge-Aubry CE, Somm E, Pernin A, et al. Adipose tissue is a regulated source of interleukin-10.Cytokine.2005;29(6): 270-274.

[19] Poonpet T, Honsawek S. Adipokines: Biomarkers for osteoarthritis?.World J Orthop.2014;5(3):319-327.

[20] Harvey S, Weisman M, O’Dell J, et al. Chondrex: new marker of joint disease. Clin Chem.1998;44(3):509-516.

[21] Blanco FJ.Osteoarthritis year in review 2014:We need more biochemical biomarkers in qualification phase.Osteoarthritis Cartilage. 2014;22(12):2025-2032.

[22] Tilg H,Moschen AR.Adipocytokines: Mediators linking adipose tissue, inflammation and immunity. Nat Rev Immunol. 2006;6(10):772-783.

[23] Thommesen L, Stunes AK, Monjo M, et al. Expression and regulation of resistin in osteoblasts and osteoclasts indicate a role in bone metabolism.J Cell Biochem.2006;99(3):824-834.

[24] Steppan CM, Bailey ST, Bhat S, et al. The hormone resistin links obesity to diabetes. Nature.2001;409(6818):307-312.

[25] Degawa-Yamauchi M,Bovenkerk JE,Juliar BE,et al.Serum resistin (FIZZ3) protein is increased in obese humans. J Clin Endocrinol Metab.2003;88(11):5452-5455.

[26] Choe JY,Bae J,Jung HY,et al.Serum resistin level is associated with radiographic changes in hand osteoarthritis: Cross-sectional study.Joint Bone Spine.2012;79(2):160-165.

[27] Massengale M, Lu B, Pan JJ, et al. Adipokine hormones and hand osteoarthritis: Radiographic severity and pain. PLoS One. 2012;7(10):e47860.

[28] Schaffler A, Ehling A, Neumann E, et al. Adipocytokines in synovial fluid[J]. JAMA.2003;290(13):1709-1710.

[29] Koskinen A,Vuolteenaho K,Moilanen T,et al.Resistin as a factor in osteoarthritis: Synovial fluid resistin concentrations correlate positively with interleukin 6 and matrix metalloproteinasesMMP-1 and MMP-3.Scand J Rheumatol. 2014;43(3):249-253.