Culture of fibroblast-like synoviocytes from rabbits with adjuvant arthritis

doi:10.3969/j.issn.2095-4344.2017.12.012

Abstract

Abstract:

BACKGROUND: Currently, fibroblast-like synoviocytes from the synovial tissue of patients with rheumatoid arthritis or adjuvant arthritis rats are isolated and cultured mainly using enzyme digestion method, but tissue explant adherent method is rarely reported in primary culture.
OBJECTIVE: To establish a culture method of fibroblast-like synoviocytes from adjuvant arthritis rabbits and to investigate cell biological characteristics.
METHODS: Rabbit models of adjuvant arthritis were prepared by injected with adjuvant at multiple sites and times, the knee synovial tissues were obtained, and fibroblast-like synoviocytes were separated and cultured by the tissue explant adherent method. Furthermore, the cell morphology was observed. The cell proliferation was measured using cell counting kit-8 assay, and the expression of Vimentin was detected by immunofluorescence.
RESULTS AND CONCLUSION: The primary cultured rabbit synoviocytes were long spindle fiber-like and coincided with the fibroblast-like synoviocytes. Moreover, cells were positive for Vimentin. In conclusion, fibroblast-like synoviocytes from rabbits with adjuvant arthritis are successfully isolated and cultured by the tissue explant method.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Arthritis, Experimental, Synovial Membrane, animals, Fibroblasts, Cell Culture Techniques, Tissue Engineering

CLC Number:

R318

Wang Li-ping, Liu Qin, Chen Fang, Chen Li-feng, Zhang Yi . Culture of fibroblast-like synoviocytes from rabbits with adjuvant arthritis[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(12): 1872-1876.

Figures/Tables 1

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