Role and significance of hydrogen peroxide-induced transforming growth factor beta1 expression in ligamentum flavum hypertrophy

doi:10.3969/j.issn.2095-4344.2017.12.011

Abstract

Abstract:

BACKGROUND: The pathogenesis of ligamentum flavum hypertrophy remains poorly understood, and the expression of transforming growth factor beta1 (TGF-β1) is increased notably. Reactive oxygen species (ROS) accumulation is associated with tissue degeneration, which may accelerate the progression of ligamentum flavum hypertrophy by upregulating TGF-β1 expression.
OBJECTIVE: To clarify the effect and significance of ROS H2O2-mediated up-regulation of TGF-β1 and collagen type I in the progress of ligamentum flavum hypertrophy.
METHODS: Ligamentum flavum was removed from a case of acquired lumbar disc herniation with normal ligamentum flavum during lumbar posterior decompression surgery, and then separated and cultured in vitro to the 4-6 generations, followed by exposure to H2O2 at various concentrations (0, 50, 100, 150, 200 μmol/L) for 72 hours. The mRNA and protein expression levels of TGF-β1 and collagen type I were detected by real-time PCR and western blot assay, respectively.
RESULTS AND CONCLUSION: Real-time quantitative PCR showed that the mRNA expression level of TGF-β1 was significantly increased in the 150 and 200 μmol/L groups (P < 0.05). The mRNA expression level of collagen type I was significantly higher in the experimental groups than that in the control group, especially in the 200 μmol/L group (P < 0.05). Western blot assay revealed that the protein expression levels of TGF-β1 and collagen type I were significantly increased in a dose-dependent manner (P < 0.05). These findings indicate that H2O2 may accelerate the progression of ligamentum flavum hypertrophy by up-regulating the expression levels of TGF-β1 and collagen type I.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Lumbar Vertebrae, Ligamentum Flavum, Free Radicals, Hydrogen Peroxide, Tissue Engineering

CLC Number:

R318

Wang Zhi-qing, Chen Xiong-sheng, Zhou Sheng-yuan, Xu Guo-feng. Role and significance of hydrogen peroxide-induced transforming growth factor beta1 expression in ligamentum flavum hypertrophy[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(12): 1867-1871.

Figures/Tables 1

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