Three-dimensional culture and differentiation of biological bone tissue and its in vivo repair effect in an animal model

doi:10.3969/j.issn.2095-4344.2017.06.003

Abstract

Abstract:

BACKGROUND: In vitro culture of tissue-engineered bone is an important method for bone repair. Three-dimensional (3D) printed bone stents combined with bioreactor culture are of significance in bone tissue engineering.
OBJECTIVE: To study the in vivo repair effect of the 3D printed biomaterial scaffold with human mesenchymal stem cells (hMSCs) cultured in bioreactor.
METHODS: The scaffold was constructed by poly(lactic-co-glycollic acid)/hydroxyapatite (PLGA/HA) via 3D printing and freeze-dying techniques, and then hMSCs were seeded onto the scaffold and cultured in bioreactors. All rabbits were numbered and divided into control (No.1 and 2), experimental 1 (No. 3 and 4) and experimental 2 (No. 5 and 6) groups, and each group had two subgroups positive and negative. The rabbit left distal femur in each group was modeled into bone defect and the single PLGA/HA scaffold, PLGA/HA scaffold carrying non-induced hMSCs were implanted in the positive and negative groups of the control group, respectively; the PLGA/HA-201 405-1 and PLGA/HA-201 405-2 carrying induced hMSCs were implanted into the positive and negative subgroups of the experimental 1 and 2 groups, respectively. Additionally, the right femur in the experimental 2 group was drilled only. The osteogenesis ability and biodegradability were determined using electron microscope, the in vivo repair was observed through CT examination, and the histopathological examination was performed after bone healing.
RESULTS AND CONCLUSION: The scaffold with topological structure suitable for cell seeding was prepared. A large number of new calcium nodules were observed under electron microscope in the experimental groups indicating overt achievement in bone healing. These results suggest that the prepared scaffold achieves a good repair effect preliminarily.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Biocompatible Materials, Cell Differentiation, Bioreactors, Bone and Bones, Tissue Engineering

CLC Number:

R318

Wang Yan1, Wang Han-zhong2, Zhang Ying1, Zhang Li-jun1, Tian Jian-ming2, Chen Xian-xiong3 . Three-dimensional culture and differentiation of biological bone tissue and its in vivo repair effect in an animal model [J]. Chinese Journal of Tissue Engineering Research, 2017, 21(6): 836-842.

Figures/Tables 5

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