Chinese Journal of Tissue Engineering Research ›› 2017, Vol. 21 ›› Issue (5): 724-729.doi: 10.3969/j.issn.2095-4344.2017.05.012

Previous Articles     Next Articles

Effect of Buyang Huanwu decoction combined with bone marrow mesenchymal stem cell transplantation on expression of integrin in a rat model of middle cerebral artery occlusion

Zhang Yun-ke1, 2, Yang Jun-hong2, Gao Feng3, Duan Feng-yang2   

  1. 1Henan University of Traditional Chinese Medicine, Zhenzhou 450008，Henan Province, China
    2First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhenzhou 450000, Henan Province, China
    3Henan Academy of Medical Pharmaceutical Sciences, Zhengzhou 450008, Henan Province, China
  • Online:2017-02-18 Published:2017-03-20
  • About author:Zhang Yun-ke, M.D., Chief physician, Henan University of Traditional Chinese Medicine，Zhenzhou 450008，Henan Province, China; First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhenzhou 450000, Henan Province, China
  • Supported by:

    the National Natural Science Foundation of China, No. 81072799/H2708; the Scientific Innovation Team Project in Henan Provincial Universities, No. 16IRTSTHN022

PDF

367

Abstract:

BACKGROUND: Previous studies have found that combined use of Buyang Huanwu decoction and bone marrow mesenchymal stem cell (BMSC) transplantation can play a synergic role against cerebral ischemia injury.
OBJECTIVE: To analyze the effect of Buyang Huanwu decoction combined with BMSC transplantation to promote angiogenesis after cerebral ischemia.
METHODS: Ninety-six Sprague-Dawley rats were randomly divided into four groups, and used to make middle cerebral artery occlusion models. In combined group, rats were given intragastrical administration of Buyang Huanwu decoction 10 mL/kg once a day, beginning at 3 days prior to modeling, and then given intragastrical administration of Buyang Huanwu decoction once at 2 hours after modeling, followed by its intragastrical administration every 12 hours. In BMSC and combined groups, BMSC suspension was injected into the rat ventricle after 2-hour cerebral ischemia/2-hour reperfusion, and then 30 minutes later, CD34 and CD45 antibodies were injected. In antibody group, CD34 and CD45 antibodies were injected. In model group, only normal saline was given. Serum αVβ3 level detection, immunohistochemical observation, Q-PCR and western blot tests were performed in the combined group at 12, 24, 36, 48 hours after reperfusion, while these indices were detected in the other three groups at 36 hours after reperfusion.
RESULTS AND CONCLUSION: (1) The level of serum αVβ3 was lower in the antibody group than the model group (P < 0.05), higher in the BMSC and combined groups than the antibody group (P < 0.05), and higher in the combined group than the BMSC group (P < 0.05). (2) Immunohistochemical findings showed that compared with the antibody group, the number of CD34 positive cells was higher in the model, BMSC and combined groups (P < 0.05). (3) Results from the Q-PCR and western blot assay showed that compared with the model group, the pFAK protein expression level was lower in the antibody and BMSC group, but FAK gene expression level had no overt changes; while the protein levels of FAK (24 hours after reperfusion) and pFAK (12 hours after reperfusion) were significantly increased in the combined group than the antibody and BMSC groups (P < 0.05,P < 0.01). Moreover, this increase exhibited a gradually rising trend with the extension of reperfusion time. To conclude, the combined use of BMSC transplantation and Buyang Huanwu decoction can reverse the effect of CD34+CD45 antibodies that lead to the decrease in the number of vascular endothelial cells and levels of integrin αVβ3 and downstream signaling molecules, thereby to promote angiogenesis in the MCAO model.

 

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Mesenchymal Stem Cells, Infarction, Middle Cerebral Artery, Drugs, Chinese Herbal, Tissue Engineering

CLC Number: