Abstract:

Abstract
BACKGROUND: Apoptosis of islet cells is closely related to the long-term hyperglycemia- and hyperlipemia-induced injuries.
OBJECTIVE: To observe the effect of Shizidaiping formula on the apoptosis and insulin secretion in MIN6 cells under the high glucose and lipid environment, and to explore the protective effect of Shizidaiping formula and the related apoptosis mechanism.
METHODS: MIN6 cells were divided into normal, model, melbine, low-, medium-and high-dose Shizidaiping formula groups. The cell activity was examined by cell counting kit-8, the insulin secretion was measured by ELISA, the rate of apoptosis was measured by Annexin V-FITC&PI and the expression levels of MEK1/2, ERK1/2 and p-ERK1/2 were examined by western blot assay.
RESULTS AND CONCLUSION: Shizidaiping formula significantly improved MIN6 cell activity under high glucose and lipid condition (P < 0.05), decreased early cell apoptosis, increased the level of insulin stimulated by low glucose in cell supernatant (P < 0.05), and improved the expression levels of MEK1/2, ERK1/2 and p-ERK1/2 (P < 0.05). These results suggest that Shizidaiping formula can protect islet cells from hyperglycemia and hyperlipemia damage by improving the activity of MIN6 cells, reducing the insulin secretion and inhibiting the apoptosis of pancreatic β cells in MIN6 cells.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Drugs, Chinese Herbal, Islets of Langerhans, Apoptosis, Tissue