Simultaneous determination of three main bioactive constituents in the in vitro fertilization medium by UHPLC-MSMS

doi:10.3969/j.issn.2095-4344.2017.02.023

Abstract

Abstract:

BACKGROUND: In the present quality control file or technique standards of in vitro fertilization medium, the indicators of the component contents and detection methods have not been clearly defined. To ensure the safety and effectiveness of these products, we should establish the quality standards as early as possible.
OBJECTIVE: To establish a method for determining the three main bioactive constituents of in vitro fertilization
medium including glucose, lactic acid sodium salt, pyruvic acid sodium salt by ultra-high performance liquid chromatography tandem mass spectrometric method (UHPLC-MSMS), and to analyze the content of each constituent.
METHODS: The UHPLC-MSMS was used, and UHPLC separation was performed on a SUPELCO Discovery HS F5-3 column (15 cm × 2.1 mm, 3 μm) in a gradient elute mode with acetonitrile and water (both containing 0.1% formic acid) as the mobile phase at a flow rate of 0.35 mL/min. The column temperature was 40℃. Mass spectrometry detection was performed with multi-reaction monitoring mode using negative electro spray ionization.
RESULTS AND CONCLUSION: The linearity was achieved in the range of 0.1-10 μg/mL (r=0.999 8) for glucose, 0.05-5 μg/mL (r=0.999 4) for lactic acid sodium salt, and 0.1-10 μg/mL (r=0.999 4) for pyruvic acid sodium salt. The recoveries were 96.4%-98.1% with relative standard deviation less than 2.8%. To conclude, the UHPLC-MSMS method is sensitive, rapid, accurate and specific, thus providing a basis for the quality standard study of in vitro fertilization medium.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Glucose, Sodium Lactate, Quality Control, Tissue Engineering

CLC Number:

R318

Huang Yuan-li, Ke Lin-nan, Wang Chun-ren, Yang Zhao-peng.

Simultaneous determination of three main bioactive constituents in the in vitro fertilization medium by UHPLC-MSMS

[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(2): 291-295.

Figures/Tables 3

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