Chitin hybrid membrane carrying cells repairs corneal epithelial injury

doi:10.3969/j.issn.2095-4344.2017.02.013

Abstract

Abstract:

BACKGROUND: Chitin has been found to be a good biomaterial, but research on chitin carrying corneal epithelial cells for rabbit corneal epithelial injury is little reported.
OBJECTIVE: To investigate the repair outcomes of chitin hybrid membrane carrying corneal epithelial cells in the rabbit corneal epithelial injury.
METHODS: Eighteen New Zealand white rabbits were enrolled and made into left corneal epithelial injury models, and then randomized into two groups and treated with chitin hybrid membrane carrying corneal epithelial cells (experimental group) and chitin hybrid membrane (control group), respectively. The damage area, histological changes and ultrastructure of the cornea were observed at 1, 3, and 7 days after implantation.
RESULTS AND CONCLUSION: Damage area of the cornea in the experimental group was significantly less than that in the control group at 1 and 3 days after implantation (P < 0.05), and the cornea in both two groups healed well at 7 days after implantation. At 7 days after implantation, in both two groups, the corneal epithelium with six layers adhered to the corneal stroma closely, which was repaired completely and regularly. Comparatively speaking, the cornea in the experimental group possessed smooth outer layer. Besides, in the experimental group, the hexagonal corneal epithelial cells arranged closely with flat surface; while the hexagonal corneal epithelial cells in the control group showed no smooth surface and gaps between cells. These results indicate that chitin hybrid membrane carrying corneal epithelial cells promotes the repair of rabbit corneal epithelial injury.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Epithelium, Cornea, Polysaccharides, Tissue Engineering

CLC Number:

R318

Lu Jing, Wu Shi-ke, Chen Guang, Zhao Yue, Li Dan.

Chitin hybrid membrane carrying cells repairs corneal epithelial injury

[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(2): 232-237.

Figures/Tables 5

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