Effects of human esophageal cancer mesenchymal stem cells on the proliferation and cell cycle of EC109 cells

doi:10.3969/j.issn.2095-4344.2017.01.007

Abstract

Abstract:

BACKGROUND: Studies have shown that biological signals such as growth factors and cytokines released from tumor cells can make mesenchymal stem cells migrate into tumor tissues, and then these cells differentiate into various stromal cells constituting a microenvironment to participate in the regulation of tumor cells.
OBJECTIVE: To investigate the effects of human esophageal cancer mesenchymal stem cells (hEC-MSCs) on the proliferation and cell cycle of human esophageal carcinoma cell line EC109.
METHODS: After isolation and purification, hEC-MSCs at 1×10⁴, 2×10⁴, 3×10⁴, 4×10⁴ were co-cultured with EC109 cells, respectively. MTT method and flow cytometry were used to measure cell proliferation and cell cycle of EC109 cells, respectively.
RESULTS AND CONCLUSION: With the increased number from 1×104 to 4×104 and increased exposure time from 24 to 72 hours, hEC-MSCs inhibited the proliferation of EC109 cells, and the survival rate of EC109cells was decreased significantly (P < 0.05). Cell cycle analysis revealed a marked increase in the EC-109 G₁ phase and a decrease in the EC-109 G₂ phase in a number-dependent manner. To conclude, hEC-MSCs inhibit the proliferation of EC109 cells and vary the cell cycle in number- and time-dependent manners.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Esophageal Neoplasms, Mesenchymal Stem Cells, Cell Proliferation, Cell Cycle, Tissue Engineering

CLC Number:

R394.2

Qian You-hui, Peng Xue-feng, Wan Yan-hui, Gong Li-hong, Wang Wei, Gu Pei-gui. Effects of human esophageal cancer mesenchymal stem cells on the proliferation and cell cycle of EC109 cells[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(1): 38-42.

Figures/Tables 3

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