Decellularized whole liver bioscaffold repairs liver injury

doi:10.3969/j.issn.2095-4344.2016.52.008

Abstract

Abstract:

BACKGROUND: Decellularized scaffolds are special for retaining the tubular structure used for nutrition transport, and providing a similar inner environment for cell growth.
OBJECTIVE: To study the preparation of the decellularized whole liver bioscaffold and to explore its repair outcomes for liver injury.
METHODS: Livers from 12 Sprague-Dawley rats were used for preparing the decellularized whole liver bioscaffold by chemical detergent-enzymes decellularized technology. Models of liver injury were established in another 24 Sprague-Dawley rats and randomized into two groups: the decellularized whole liver bioscaffold was implanted into the rat liver lesions in experimental group, and controls were given the injection of normal saline. Thirty days later, the serum levels of alanine aminotransferase and glutamic-oxaloacetic transaminase were detected, and liver tissues were removed for hematoxylin-eosin staining.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that extracellular matrix-like structures existed in the decellularized bioscaffold; cell components were completely removed from the liver, the collagen fibers in the scaffold arranged regularly and were not dissolved under electron microscope. The serum levels of alanine aminotransferase and glutamic-oxaloacetic transaminase in the experimental group were significantly lower than those in the control group (P < 0.05). Hematoxylin-eosin staining showed a large number of blue-stained and dense distributed nuclei, and pink distribution of collagen fibers that had no overt breakages in the control group, while pink and dense structures in the experimental group. These results suggest that the decellularized whole liver bioscaffold is easy to obtain, and can promote the injured liver repair.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Liver, Artificial, Transaminases, Tissue Engineering

CLC Number:

R318

Zhang Qing-feng, Li Zi-rong.

Decellularized whole liver bioscaffold repairs liver injury

[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(52): 7809-7814.

Figures/Tables 4

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