Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (52): 7765-7772.doi: 10.3969/j.issn.2095-4344.2016.52.002

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Physiochemical properties and cytotoxicity of silk fibroin based calcium phosphate cement incorporated with N-acetylcysteine

Feng Tao, Pi Bin, Jiang Lei, Li Bin, Lu Ying-jie, Yang Hui-lin, Zhu Xue-song
  

  1. Department of Orthopaedics, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China
  • Received:2016-09-26 Online:2016-12-16 Published:2016-12-16
  • Contact: Zhu Xue-song, Department of Orthopedics, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China
  • About author:Feng Tao, Master, Physician, Department of Orthopedics, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China Pi Bin, M.D., Physician, Department of Orthopedics, the First Affiliated Hospital of Soochow University, Suzhou 215006, Jiangsu Province, China Feng Tao and Pi Bin contributed equally to this work.
  • Supported by:

    the National 863 Program of China, No. 2015AA020316; the National Natural Science Foundation of China, No. 81171689, 81301559; the Natural Science Foundation of Jiangsu Province, No. SBK201341421

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Abstract:

BACKGROUND: Calcium phosphate cements (CPCs) possess the bio-degradation and osteoconduction, and its final hydration product, hydroxyapatite, is the main inorganic constituent of bones. However, its poor mechanical property makes it unable to be used for repairing weight-bearing bone defects.
OBJECTIVE: To develop a kind of bioactive bone cements with decent biomechanical property and biocompatibility.
METHODS: 6% silk fibroin aqueous solutions containing different concentrations of N-acetylcysteine (0, 10 and 25 mmol/L) were prepared. Each cement sample was prepared by mixing the curing liquid and α-tricalcium phosphate powder with the ratio of 0.4 mL: 1 g; α-tricalcium phosphate powder mixed with ddH2O as control group. The compressive strength, setting time of the cements were measured. The crystal components of the cements were characterized using X-ray diffraction and the microstructure was observed using scanning electron microscope. MC3T3-E1 cells were seeded onto the material in each group, and cell morphology was observed under scanning electron microscope at 24 hours. MC3T3-E1 cells were cultured in the extract of each material, cell proliferation was detected at 1, 3, 5 and 7 days, and the lactate dehydrogenase level was detected at 1 and 3 days.
RESULTS AND CONCLUSION: X-ray diffraction and scanning electron microscope showed that the final hydration products of α-tricalcium phosphate in all specimens were hydroxyapatite. When the concentration of N-acetylcysteine was 25 mmol/L, the compressive strength of the material reached (49.39±1.68) MPa, with the initial setting time of (21.77±1.07) minutes and the final setting time of (31.88±1.69) minutes. There was no significant difference in cell morphology among cements. These results suggest that the cement containing N-acetylcysteine exhibites good biocompatibility and high mechanical strength.

Key words: Calcium Phosphates, Silk, Cysteamine, Tissue Engineering

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