5-Azacytidine induces differentiation of bone marrow mesenchymal stem cells into myocardial cells

doi:10.3969/j.issn.2095-4344.2016.50.003

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells can be differentiated into myocardial cells induced by 5-azacytidine in vitro, which provides an opportunity for cell transplantation in the treatment of heart disease.
OBJECTIVE: To study the induction effects of 5-azacytidine at different concentrations on the myocardial differentiation of bone marrow mesenchymal stem cells in rats.
METHODS: Passage 3 bone marrow mesechymal stem cells were incubated with DMEM containing 0, 5, 10, 20 μmol/L 5-azacytidine for 24 hours, and then the induced medium was replaced by DMEM containing 5% fetal bovine serum for subsequent 28-day culture. Afterwards, expression of cardiac troponin I was detected by immunocytochemical method.
RESULTS AND CONCLUSION: Cell death occurred at 24 hours after 5-azacytidine induction, which was more obvious in the 20 μmol/L group than the 5 and 10 μmol/L groups. The positive expression of cardiac troponin I was significantly lower in the 5 μmol/L group than the 10 and 20 μmol/L groups (P < 0.05), but there was no significant difference between the 10 and 20 μmol/L groups (P > 0.05). These experimental findings indicate that 5-azacytidine can induce the differentiation of rat bone marrow mesenchymal stem cells into myocardial cells in vitro, and its optimal concentration is 10 μmol/L.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Azacitidine, Myocytes, Cardiac, Cell Differentiation, Troponin I, Tissue Engineering

CLC Number:

R394.2

Jia Xiu-li, Li Chang-ping. 5-Azacytidine induces differentiation of bone marrow mesenchymal stem cells into myocardial cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(50): 7469-7474.

Figures/Tables 2

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