Effects of FAT10 gene silencing on apoptosis of human esophageal cancer cells and the characteristics of cancer stem cells

doi:10.3969/j.issn.2095-4344.2016.50.001

Abstract

Abstract:

BACKGROUND: Human leukocyte antigen F-associated transcription factor 10 (FAT10) is highly expressed in many tumor cells like colon cancer cells, but its relationship with esophageal cancer is less reported.
OBJECTIVE: To investigate the effects of siRNA interference technique on the invasion, apoptosis and the characteristics of EC9706 cells, a human esophageal cancer cell line.
METHODS: siRNA sequence was designed and synthesized according to the FAT10 mRNA encoding sequence, and the EC9706 cells were transiently transfected. EC9706 cells were divided into three groups: siRNA FAT10 group, negative control group, and blank control group. The expression levels of bcl-2 and FAT10 were detected by RT-PCR and western blot assay, respectively. Cell counting kit-8 assay was used to measure the proliferation of cells in vitro. Flow cytometry was used to observe the changes of cell cycle, cell apoptosis and the expression of CD44⁺CD133⁺. TUNEL staining was used to detect the apoptosis of the cells. Cell invasion in vitro was detected by Transwell invasion assay.
RESULTS AND CONCLUSION: RT-PCR and western blot findings showed that compared with the negative control group and blank control group, the expression levels of bcl-2 and FAT10 mRNA and protein were significantly decreased in the siRNA FAT10 group (P < 0.05); the percentage of CD44⁺CD133⁺ cells was decreased significantly (P < 0.05); and significantly increased apoptosis rate, and decreased cell proliferation and invasion were also found in the siRNA FAT10 group (P < 0.05). In conclusion, the specific silencing of FAT10 gene can reduce the invasion of esophageal cancer cells, inhibit cell proliferation, reduce bcl-2 expression, and increase the apoptosis rate. Meanwhile, the proportion of CD44⁺CD133⁺ cells is decreased.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, RNA Interference, Neoplastic Stem Cells, Apoptosis, Esophageal Neoplasms, Tissue Engineering

CLC Number:

R394.2

Wang Zhi-qiang, Zhang Jian-jun. Effects of FAT10 gene silencing on apoptosis of human esophageal cancer cells and the characteristics of cancer stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(50): 7453-7459.

Figures/Tables 2

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