Overexpression of glycogen synthase kinase 3beta in the treatment of myocardial infarction with cardiac stem cell transplantation

doi:10.3969/j.issn.2095-4344.2016.41.019

Abstract

Abstract:

BACKGROUND: The mechanism and effect of glycogen synthase kinase 3β (GSK-3β) in the differentiation of cardiac stem cells into cardiomyocytes are still unclear, although GSK-3β is closely related to the life activities of cells.
OBJECTIVE: To investigate the changes of GSK-3β expression in the treatment of myocardial infarction in rats undergoing cardiac stem cell transplantation.
METHODS: The isolation and culture of cardiac stem cells were performed in 10 neonatal rats. Lentivirus overexpressing GSK-3β or LacZ (control) was constructed and transferred into cardiac stem cells. Animal model of myocardial infarction was made in 30 Sprague-Dawley rats. Six weeks after model preparation, rat models were assigned into GSK-3β, LacZ or PBS group. GSK-3β or LacZ overexpressing cardiac stem cell solution or PBS in equal volume was injected into the rat myocardium, respectively. Four weeks after transplantation, the cardiac function and myocardial collagen production in rats were detected and compared.
RESULTS AND CONCLUSION: Compared with the other two groups, the left ventricular ejection fraction was significantly higher, and the left ventricular end diastolic diameter was significantly lower in the GSK-3β group (P < 0.05). Hydroxyproline content, type I collagen mRNA, and type III collagen mRNA expression were significantly lower in the GSK-3β group than the other two groups (P < 0.05). Findings from Masson staining showed that the content of blue-stained collagen was significantly lower in the GSK-3β group than the LacZ group. Moreover, lowest myocardial infarction size was found in the GSK-3β group (P < 0.05). All these experimental findings show that GSK-3 overexpression plays a positive role in promoting the therapeutic effect of cardiac stem cell transplantation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Myocardial Infarction, Myocytes, Cardiac, Glycogen Synthase Kinases, Tissue Engineering

CLC Number:

R394.2

Zhao Cui-hua, Li Yan-ming, Zhong Xiao-ming, He Rui-li, Cheng Guan-chang. Overexpression of glycogen synthase kinase 3beta in the treatment of myocardial infarction with cardiac stem cell transplantation[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(41): 6203-6208.

Figures/Tables 2

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