Biological characteristics of adipose-derived stem cells derived from renal fat capsule and groin in vitro

doi:10.3969/j.issn.2095-4344.2016.41.018

Abstract

Abstract:

BACKGROUND: Adipose-derived stem cells have different sources, but it is unclear whether these cells from different sources have difference in their biological properties.
OBJECTIVE: To detect the in vitro proliferation and chondrogenic differentiation of adipose-derived stem cells derived from renal fat capsule and groin.
METHODS: Adipose-derived stem cells from renal fat capsule and groin of rats were isolated, cultured and identified. MTT assay was used to detect in vitro proliferation ability of these cells. Passage 3 cells were under chondrogenic induction for 2 weeks. After induction, the expression of type II collagen was observed by immunofluorescence detection, and RT-PCR was employed to detect the expression levels of Aggrecan and type II collagen mRNA in the two groups.
RESULTS AND CONCLUSION: After primary culture and passage, adipose-derived stem cells from the renal fat capsule and groin of rats exhibited similar morphology, and over 95% of cells expressed CD44 in the two groups. Adipose-derived stem cells from two sources showed an S-shaped growth curve in vitro and were positive for type II collagen. After RT-PCR detection, the expression levels of Aggrecan and type II collagen mRNA had no difference in adipose-derived stem cells from renal fat capsule and groin (P > 0.05). Experimental results show that adipose-derived stem cells from both renal fat capsule and groin exhibit stable growth, rapid proliferation and chondrogenic differentiation under orient induction in vitro, indicating there is no difference between these cells from two sources.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Adipose Tissue, Kidney, Groin, Tissue Engineering

CLC Number:

R394.2

Zhu Yong-sheng, Deng Qing-fu, Li Jun. Biological characteristics of adipose-derived stem cells derived from renal fat capsule and groin in vitro[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(41): 6197-6202.

Figures/Tables 2

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