Interleukin 8 is involved in the invasion and metastasis of CD133+ hepatocellular carcinoma stem cells

doi:10.3969/j.issn.2095-4344.2016.41.010

Abstract

Abstract:

BACKGROUND: Interleukin-8 is an important inflammatory chemokine that plays an important role in the regulation of tumor cell proliferation and angiogenesis.
OBJECTIVE: To investigate the effect of interleukin-8 on the invasion and metastasis of CD133⁺ hepatocellular carcinoma stem cells.
METHODS: After isolation and culture of MHCC97-H cell lines, CD133⁺/CD133^-MHCC97-H cells were sorted using immunomagnetic beads. CD133 expression was detected using flow cytometry, and interleukin-8 level in supernatant was measured using ELISA method. Cloning efficiency, tumorigenic capacity, cell migration and invasion ability were detected through colony formation assay, tumorigenesis experiment in nude mice, and Transwell detection. Additionally, other cells were neutralized using interleukin-8 neutralizing antibody. Measurement results were compared between cells undergoing different treatments.
RESULTS AND CONCLUSION: The CD133 level, interleukin-8 level, cloning efficiency and cell membrane permeability of CD133⁺MHCC97-H cells were significantly higher than those of CD133^-MHCC97-H cells (P < 0.05). Transplantation of CD133⁺MHCC97-H cells at 1×10⁶/L and 1×10⁷/L resulted in subcutaneous tumors in some mice, whereas no subcutaneous tumors appeared in mice undergoing transplantation of CD133^-MHCC97-H cells at the same concentrations. After interleukin-8 neutralizing antibody treatment, the CD133 level, interleukin-8 level, and cloning efficiency of CD133⁺/CD133^-MHCC97-H cells were significantly decreased (P < 0.05), especially in the CD133⁺MHCC97-H cells (P < 0.01); the migration and invasion ability and cell membrane permeability of CD133⁺MHCC97-H cells were significantly reduced (P < 0.05), but these changes were not obvious in CD133^-MHCC97-H cells (P > 0.05). These results show that interleukin-8 could be specifically involved in the invasion and metastasis of CD133⁺MHCC97-H cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Liver Neoplasms, Neoplastic Stem Cells, Antigens, CD, Neoplasm Invasiveness, Neoplasm Metastasis, Tissue Engineering

CLC Number:

R394.2

Wen Li-hong, Hu Wen-jie, Ye Heng-xi, Xiang Wei-dong. Interleukin 8 is involved in the invasion and metastasis of CD133+ hepatocellular carcinoma stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(41): 6145-6150.

Figures/Tables 1

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